sphingosine 1 phosphate phosphatase 1 (SGPP1) (NM_030791) Human 3' UTR Clone

SKU SC216952

3' UTR clone of sphingosine-1-phosphate phosphatase 1 (SGPP1) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: sphingosine 1 phosphate phosphatase 1
• Synonyms: SPP-1; SPPase1
• ACCN: NM_030791
• Insert Size: 1919 bp
• Sequence Data:
  Insert Sequence

  >SC216952 3’UTR clone of NM_030791 ; The sequence shown below is from the reference sequence of NM_030791. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; TACATATTTTTCTTTATTGGTATCTCTTGATGGAGAAGTATTGTTTATGATAAGAAAGGAGGGTATCAG; TTACTGATACCCAAAAATATATTCCAGGTAAAGCCAGGTCAGAATTAGGCTTTTGCAGGAATTTAACTT; AAATAATTATTTAAGTAAATTCATAAGAGTCAGTGCATTTTATCATTGCACATCCAGATACTGTTTTAG; GTGAGCTGAGCTATTTCGTTACTGAGAAAATGATAAGTATCCATTTAGAATGTTCATGTAATGTTTGGA; GAGTTTTGTATTATGGATTCAACTTATATATAATATATATAAGGTACATAATATGCAATTATATATCTG; ATATATATTATATATAAAATAATATACCTAAGTGTTTTTTTAAACTATGGGAGTGTATTATAAAATCAA; TAATAATATGCAAACAGTTGTGCCTGTGTATTTGAACTACATACAGGTATGTTGTTATTAACAGATATA; TTTAACATTTATTTACTATGGGAGCCATTTCCTAATTGAATTGCGTAATTACTAGACCAGAATGCATAT; GCTACTATACATTGATTTACTGCCTGTTTTTACACTGCCATTACCTTTCATGTTATCCATGATGTTGAA; CATGGGAGGCATTTTTAATGGACCAAATTTTTAGAAAAATTAGTTTTTGGATTTTTTTTTAAGTTCTGT; ATACTGTTTTGAATGAACTTTATTTGCAGCTATTCTGGATGTCAGTTTAATAATTCAGGTACTGAATTT; TATTGCTTGCTAATTGTGCCTTTCCGTTGCTAAATTAAGAAATGCTATGTGTACTGTGATGTAAAAATA; AGACATTAACTTTATCTTAAGTTACATATAATCAAGCAAATATTTTAAAAAATGTATGTCAGGGTGACA; GGCATAAAAGTAAATTACCCTTAACTCTTTAGCCTTTATTTAGAAAGAAATGTGGAATGGGCTAAACTT; TCTCACCAATTTATTAGAAAGCTAAATGGATAAATACTTCCCTTTTTGTAGATATTTTTCTGTGCATTG; TAAGTTATAGGAGCAGGACATATTTTTTGTACATTGTCTTGATTGTTTATACTGCAGGTAGGTATTTCC; AGTGAATGGAAACGTTGAAATTTATAGATGGGACAATGCACATACTTCATATGTAAACAAAGCATTTTT; CTAAATAATTTGGAAAGGATTGGATTTAGCACCGTTAACTTTTAATACCCATTTATTGGCTATTTAAGG; TAAATTATAAAACCTAAAATTAGTATCAGTATTTTAGATCCTATTATTTTTTCAACTTTCTATTATTTT; CACAGTGGAAAATTTGCGTATAGTATTAAACTATTCTTTTTTCTGTCTACCTTTATATTTTGAAGTGTG; GTTGTACTTACGTTAATAGTAAGTGTATTTGTAGAAAGTTTTTCATAAAGAAGAGATTAACTTTCCATA; TAAATAAATACCAGGTATGAAATTACTGATTTTAGTGCAATATCTGTATGATTATCCAGAACTATCACT; TCAGATGTTTCTATTTTGGGCACCATAACTATTTGAGCTCATATTTTTATAATTCTGATTGAGAATTTA; GGGGCATGGTAATTTTTACCAAAACTGAAGCTGGTAATTTTAAGTAAAGCAACTAAATTTACCCTTTAC; TTATTAGTTAAAAGGAAACCAAGGCATGTAGGAAAATATTGGTACGTTGGAAAATGTGAAAAGAGCTTT; ACTTTCAAAATGTATGTTTAATGAATTGAATTTTAGGGACCGTTTCCCATAATTTGGGCCTATGCTATC; CTGTTGATTTTTATGTCTAGCTTAAAAGGTATTTGAAAATGTTCCATTTGTGTTTTATTATTTTCTGTC; CTATGACAGTTCTATGCTAAATGTATTTTAAAATAAAAGCCAAATTGAAGATTAAA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_030791.4
• Locus ID: 81537
• Summary: Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite that regulates diverse biologic processes. SGPP1 catalyzes the degradation of S1P via salvage and recycling of sphingosine into long-chain ceramides (Mandala et al., 2000 [PubMed 10859351]; Le Stunff et al., 2007 [PubMed 17895250]).[supplied by OMIM, Jun 2009]