Filamin B (FLNB) (NM_001457) Human 3' UTR Clone

SKU SC214898

3' UTR clone of filamin B beta (FLNB) transcript variant 2 for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: Filamin B
• Synonyms: ABP-278; ABP-280; AOI; FH1; FLN-B; FLN1L; LRS1; SCT; TABP; TAP
• ACCN: NM_001457
• Insert Size: 1519 bp
• Sequence Data:
  Insert Sequence

  >SC214898 3’UTR clone of NM_001457 ; The sequence shown below is from the reference sequence of NM_001457. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; GGCAGCCCTTTTCATGTCACAGTGCCTTAAAACAGTTTTCTCAAATCCTGGAGAGAGTTCTTGTGGTTG; CTTTTGTTGCTTGTTTGTAATTCATTTTATACAAAGCCCTCCAGCCTGTTTGTGGGGCTGAAACCCCAT; CCCTAAAATATTGCTGTTGTAAAATGCCTTCAGAAATAAGTCCTAGACTGGACTCTTGAGGGACATATT; GGAGAATCTTAAGAAATGCAAGCTTGTTCAGGGGGCTGAGAAGATCCTGAGTACACTAGGTGCAAACCA; GAACTCTTGGTGGAACAGACCAGCCACTGCAGCAGACAGACCAGGAACACAATGAGACTGACATTTCAA; AAAAACAAAACTGGCTAGCCTGAGCTGCTGGTTCACTCTTCAGCATTTATGAAACAAGGCTAGGGGAAG; ATGGGCAGAGAAAAAGGGGACACCTAGTTTGGTTGTCATTTGGCAAAGGAGATGACTTAAAATCCGCTT; AATCTCTTCCAGTGTCCGTGTTAATGTATTTGGCTATTAGATCACTAGCACTGCTTTACCGCTCCTCAT; CGCCAACACCCCCATGCTCTGTGGCCTTCTTACACTTCTCAGAGGGCAGAGTGGCAGCCGGGCACCCTA; CAGAAACTCAGAGGGCAGAGTGGCAGCCAGGCCCACATGTCTCTCAAGTACCTGTCCCCTCGCTCTGGT; GATTATTTCTTGCAGAATCACCACACGAGACCATCCCGGCAGTCATGGTTTTGCTTTAGTTTTCCAAGT; CCGTTTCAGTCCCTTCCTTGGTCTGAAGAAATTCTGCAGTGGCGAGCAGTTTCCCACTTGCCAAAGATC; CCTTTTAACCAACACTAGCCCTTGTTTTTAACACACGCTCCAGCCCTTCATCAGCCTGGGCAGTCTTAC; CAAAATGTTTAAAGTGATCTCAGAGGGGCCCATGGATTAACGCCCTCATCCCAAGGTCCGTCCCATGAC; ATAACACTCCACACCCGCCCCAGCCAACTTCATGGGTCACTTTTTCTGGAAAATAATGATCTGTACAGA; CAGGACAGAATGAAACTCCTGCGGGTCTTTGGCCTGAAAGTTGGGAATGGTTGGGGGAGAGAAGGGCAG; CAGCTTATTGGTGGTCTTTTCACCATTGGCAGAAACAGTGAGAGCTGTGTGGTGCAGAAATCCAGAAAT; GAGGTGTAGGGAATTTTGCCTGCCTTCCTGCAGACCTGAGCTGGCTTTGGAATGAGGTTAAAGTGTCAG; GGACGTTGCCTGAGCCCAAATGTGTAGTGTGGTCTGGGCAGGCAGACCTTTAGGTTTTGCTGCTTAGTC; CTGAGGAAGTGGCCACTCTTGTGGCAGGTGTAGTATCTGGGGCGAGTGTTGGGGGTAAAAGCCCACCCT; ACAGAAAGTGGAACAGCCCGGAGCCTGATGTGAAAGGACCACGGGTGTTGTAAGCTGGGACACGGAAGC; CAAACTGGAATCAAACGCCGACTGTAAATTGTATCTTATAACTTATTAAATAAAACATTTGCTCCGTAAA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001457.4
• Locus ID: 2317
• Summary: This gene encodes a member of the filamin family. The encoded protein interacts with glycoprotein Ib alpha as part of the process to repair vascular injuries. The platelet glycoprotein Ib complex includes glycoprotein Ib alpha, and it binds the actin cytoskeleton. Mutations in this gene have been found in several conditions: atelosteogenesis type 1 and type 3; boomerang dysplasia; autosomal dominant Larsen syndrome; and spondylocarpotarsal synostosis syndrome. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Nov 2009]