HB EGF (HBEGF) (NM_001945) Human 3' UTR Clone

SKU SC214809

3' UTR clone of heparin-binding EGF-like growth factor (HBEGF) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: HB EGF
• Synonyms: DTR; DTS; DTSF; HEGFL
• ACCN: NM_001945
• Insert Size: 1486 bp
• Sequence Data:
  Insert Sequence

  >SC214809 3’UTR clone of NM_001945 ; The sequence shown below is from the reference sequence of NM_001945. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; GTGAAGTTGGGCATGACTAATTCCCACTGAGAGAGACTTGTGCTCAAGGAATCGGCTGGGGACTGCTAC; CTCTGAGAAGACACAAGGTGATTTCAGACTGCAGAGGGGAAAGACTTCCATCTAGTCACAAAGACTCCT; TCGTCCCCAGTTGCCGTCTAGGATTGGGCCTCCCATAATTGCTTTGCCAAAATACCAGAGCCTTCAAGT; GCCAAACAGAGTATGTCCGATGGTATCTGGGTAAGAAGAAAGCAAAAGCAAGGGACCTTCATGCCCTTC; TGATTCCCCTCCACCAAACCCCACTTCCCCTCATAAGTTTGTTTAAACACTTATCTTCTGGATTAGAAT; GCCGGTTAAATTCCATATGCTCCAGGATCTTTGACTGAAAAAAAAAAAGAAGAAGAAGAAGGAGAGCAA; GAAGGAAAGATTTGTGAACTGGAAGAAAGCAACAAAGATTGAGAAGCCATGTACTCAAGTACCACCAAG; GGATCTGCCATTGGGACCCTCCAGTGCTGGATTTGATGAGTTAACTGTGAAATACCACAAGCCTGAGAA; CTGAATTTTGGGACTTCTACCCAGATGGAAAAATAACAACTATTTTTGTTGTTGTTGTTTGTAAATGCC; TCTTAAATTATATATTTATTTTATTCTATGTATGTTAATTTATTTAGTTTTTAACAATCTAACAATAAT; ATTTCAAGTGCCTAGACTGTTACTTTGGCAATTTCCTGGCCCTCCACTCCTCATCCCCACAATCTGGCT; TAGTGCCACCCACCTTTGCCACAAAGCTAGGATGGTTCTGTGACCCATCTGTAGTAATTTATTGTCTGT; CTACATTTCTGCAGATCTTCCGTGGTCAGAGTGCCACTGCGGGAGCTCTGTATGGTCAGGATGTAGGGG; TTAACTTGGTCAGAGCCACTCTATGAGTTGGACTTCAGTCTTGCCTAGGCGATTTTGTCTACCATTTGT; GTTTTGAAAGCCCAAGGTGCTGATGTCAAAGTGTAACAGATATCAGTGTCTCCCCGTGTCCTCTCCCTG; CCAAGTCTCAGAAGAGGTTGGGCTTCCATGCCTGTAGCTTTCCTGGTCCCTCACCCCCATGGCCCCAGG; CCCACAGCGTGGGAACTCACTTTCCCTTGTGTCAAGACATTTCTCTAACTCCTGCCATTCTTCTGGTGC; TACTCCATGCAGGGGTCAGTGCAGCAGAGGACAGTCTGGAGAAGGTATTAGCAAAGCAAAAGGCTGAGA; AGGAACAGGGAACATTGGAGCTGACTGTTCTTGGTAACTGATTACCTGCCAATTGCTACCGAGAAGGTT; GGAGGTGGGGAAGGCTTTGTATAATCCCACCCACCTCACCAAAACGATGAAGTTATGCTGTCATGGTCC; TTTCTGGAAGTTTCTGGTGCCATTTCTGAACTGTTACAACTTGTATTTCCAAACCTGGTTCATATTTAT; ACTTTGCAATCCAAATAAAGATAACCCTTATTCCATA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001945.3
• Locus ID: 1839
• Summary: Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.[UniProtKB/Swiss-Prot Function]