Stromal interaction molecule 1 (STIM1) (NM_003156) Human 3' UTR Clone

CAT#: SC214586

3' UTR clone of stromal interaction molecule 1 (STIM1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: STIM1
• Synonyms: D11S4896E; GOK; IMD10; STRMK; TAM; TAM1
• ACCN: NM_003156
• Insert Size: 1440 bp
• Sequence Data:
  Insert Sequence

  >SC214586 3’UTR clone of NM_003156
  The sequence shown below is from the reference sequence of NM_003156. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AAAATCTTTAAGAAGCCTCTTAAGAAGTAGGCAGGATGGGGTGGCAGTAAAGGGACAGCTTGTCCTTCC
  CTGGGTGTTCTGTCTCTCCTTCCCTCCCTTCCTTCAAGATAACTGGCCCCAAGAGTGGGGCATGGGAAG
  GGCTGGTCCAGGGGTCTGGGCACTGTACATACCTGCCCCCTCATCCTTGGGTCCTTCATTATTATTTAT
  TAACTGACCACCATGGCCTGCCTGCCCTGCCTCCGTCCCAACCATGGGCTGCTGCTGTCACTCCCTCTC
  CACTTCAGTGCATGTCTTAGTTGCTGTTCCCTCAGCTCCCAGCTCCACCTCTGGGGTTCAGCTTCTGTC
  TCTGCTGTCCCAGTTTTGAGGTTTGGTTTCTTGTTTCTGTCTCTTGCTTTCGGGCTCCTCCCTCCCACC
  ACTCCCCAACTTCCCCTAGCAGTTGCAGGGAAGATAGGACGAGTAGCTTCTGACATGTGTGCCTCAGAT
  CTGTTCCACCCCACTCACAGTGGTTCTGTTTGCTCCAGACTGGGGCTAGGGCCTAATCTTTGAAGTTTG
  TTCTTTGGTATTGATGTGGGTCAGAAGGAGCCTCATCCTAATCTCACTCAGGCCTCCAGGGATCCATGG
  GGGAGTGAAACCAATTCTCAGAGAACAACCCACCAGAGACTTTTAAAGAGAGGCCAGGCTTGGGAATGG
  GTTGGGAGAGGCATCTGTTCATTGGAGCATGAGTGGATGCCAGAACTGTAGGTTATAAGGCAGTCACTT
  TTTCTCTCTACTCCCACCCACACCTGCCTCCCTCTTACCCCTGCTCCCCCACACTGCAGGAGGATTTGT
  CTCTAAGAGGTGCTGCCCCAAAGCTCCCCAAGCATCAATACTCCTAGGGCTCAGGACAAGTGGCTCCCC
  TGGCCAGGAGAGCCACAGCCATGATACAGGGCTCTTATGGAGCCCTGGAGTTGTTGGGCAAGGATGCTG
  TCATTTTTTGAACCAAAAGACAAACAGGTTAAAAGGAAAAAAAGTAATCTGAATTTCCCAAGTGCCTAC
  GCTGCATATTCCCCTTGTTAGATCCCATTTTCATGTTACTTTGTAGCCTTGGCCAGAGGCTCAAAAAGG
  ACACAACCAGTTTGGGGAAGGGGTGGCTAAGGAAGATGGTATAGGTGAAGGCGGCTGTGTGACCACTTT
  CCCCCACCCTTCCCACCCTCTAGACAACTCTCTCCCTTACCTGTTTTTGCTATGGCTGTAAAGGTATTT
  TTCCTCTGCCCCACTCCCTGCCATACCTTTATCCTGGGATCCTATTTTGGGCCTGGGGTGGGTATACCT
  GGGGCTGGTCTTAGGAGGGTGCTAGGCTGCAGACTGCCTTGTACTCCCTGGACACCCTCAAATGGGGTT
  TTCTGTGTTATTTCATAAAATTCTTTGAAGTCCAATAAAGCATGTAGGAGATTTTAACCA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003156.4
• Summary: This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. Mutations in this gene are associated with fatal classic Kaposi sarcoma, immunodeficiency due to defects in store-operated calcium entry (SOCE) in fibroblasts, ectodermal dysplasia and tubular aggregate myopathy. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]
• Locus ID: 6786
• MW: 52.9