Stromal interaction molecule 1 (STIM1) (NM_003156) Human 3' UTR Clone

SKU SC214586

3' UTR clone of stromal interaction molecule 1 (STIM1) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: Stromal interaction molecule 1
• Synonyms: D11S4896E; GOK; IMD10; STRMK; TAM; TAM1
• ACCN: NM_003156
• Insert Size: 1440 bp
• Sequence Data:
  Insert Sequence

  >SC214586 3’UTR clone of NM_003156 ; The sequence shown below is from the reference sequence of NM_003156. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; AAAATCTTTAAGAAGCCTCTTAAGAAGTAGGCAGGATGGGGTGGCAGTAAAGGGACAGCTTGTCCTTCC; CTGGGTGTTCTGTCTCTCCTTCCCTCCCTTCCTTCAAGATAACTGGCCCCAAGAGTGGGGCATGGGAAG; GGCTGGTCCAGGGGTCTGGGCACTGTACATACCTGCCCCCTCATCCTTGGGTCCTTCATTATTATTTAT; TAACTGACCACCATGGCCTGCCTGCCCTGCCTCCGTCCCAACCATGGGCTGCTGCTGTCACTCCCTCTC; CACTTCAGTGCATGTCTTAGTTGCTGTTCCCTCAGCTCCCAGCTCCACCTCTGGGGTTCAGCTTCTGTC; TCTGCTGTCCCAGTTTTGAGGTTTGGTTTCTTGTTTCTGTCTCTTGCTTTCGGGCTCCTCCCTCCCACC; ACTCCCCAACTTCCCCTAGCAGTTGCAGGGAAGATAGGACGAGTAGCTTCTGACATGTGTGCCTCAGAT; CTGTTCCACCCCACTCACAGTGGTTCTGTTTGCTCCAGACTGGGGCTAGGGCCTAATCTTTGAAGTTTG; TTCTTTGGTATTGATGTGGGTCAGAAGGAGCCTCATCCTAATCTCACTCAGGCCTCCAGGGATCCATGG; GGGAGTGAAACCAATTCTCAGAGAACAACCCACCAGAGACTTTTAAAGAGAGGCCAGGCTTGGGAATGG; GTTGGGAGAGGCATCTGTTCATTGGAGCATGAGTGGATGCCAGAACTGTAGGTTATAAGGCAGTCACTT; TTTCTCTCTACTCCCACCCACACCTGCCTCCCTCTTACCCCTGCTCCCCCACACTGCAGGAGGATTTGT; CTCTAAGAGGTGCTGCCCCAAAGCTCCCCAAGCATCAATACTCCTAGGGCTCAGGACAAGTGGCTCCCC; TGGCCAGGAGAGCCACAGCCATGATACAGGGCTCTTATGGAGCCCTGGAGTTGTTGGGCAAGGATGCTG; TCATTTTTTGAACCAAAAGACAAACAGGTTAAAAGGAAAAAAAGTAATCTGAATTTCCCAAGTGCCTAC; GCTGCATATTCCCCTTGTTAGATCCCATTTTCATGTTACTTTGTAGCCTTGGCCAGAGGCTCAAAAAGG; ACACAACCAGTTTGGGGAAGGGGTGGCTAAGGAAGATGGTATAGGTGAAGGCGGCTGTGTGACCACTTT; CCCCCACCCTTCCCACCCTCTAGACAACTCTCTCCCTTACCTGTTTTTGCTATGGCTGTAAAGGTATTT; TTCCTCTGCCCCACTCCCTGCCATACCTTTATCCTGGGATCCTATTTTGGGCCTGGGGTGGGTATACCT; GGGGCTGGTCTTAGGAGGGTGCTAGGCTGCAGACTGCCTTGTACTCCCTGGACACCCTCAAATGGGGTT; TTCTGTGTTATTTCATAAAATTCTTTGAAGTCCAATAAAGCATGTAGGAGATTTTAACCA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003156.4
• Locus ID: 6786
• Summary: This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. Mutations in this gene are associated with fatal classic Kaposi sarcoma, immunodeficiency due to defects in store-operated calcium entry (SOCE) in fibroblasts, ectodermal dysplasia and tubular aggregate myopathy. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]