PIM1 (NM_002648) Human 3' UTR Clone

SKU SC214258

3' UTR clone of pim-1 oncogene (PIM1) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: PIM1
• Synonyms: PIM
• ACCN: NM_002648
• Insert Size: 1367 bp
• Sequence Data:
  Insert Sequence

  >SC214258 3’UTR clone of NM_002648 ; The sequence shown below is from the reference sequence of NM_002648. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; CACAGCCTGTCGCCGGGGCCCAGCAAATAGCAGCCTTTCTGGCAGGTCCTCCCCTCTCTTGTCAGATGC; CCGAGGGAGGGGAAGCTTCTGTCTCCAGCTTCCCGAGTACCAGTGACACGTCTCGCCAAGCAGGACAGT; GCTTGATACAGGAACAACATTTACAACTCATTCCAGATCCCAGGCCCCTGGAGGCTGCCTCCCAACAGT; GGGGAAGAGTGACTCTCCAGGGGTCCTAGGCCTCAACTCCTCCCATAGATACTCTCTTCTTCTCATAGG; TGTCCAGCATTGCTGGACTCTGAAATATCCCGGGGGTGGGGGGTGGGGGTGGGTCAGAACCCTGCCATG; GAACTGTTTCCTTCATCATGAGTTCTGCTGAATGCCGCGATGGGTCAGGTAGGGGGGAAACAGGTTGGG; ATGGGATAGGACTAGCACCATTTTAAGTCCCTGTCACCTCTTCCGACTCTTTCTGAGTGCCTTCTGTGG; GGACTCCGGCTGTGCTGGGAGAAATACTTGAACTTGCCTCTTTTACCTGCTGCTTCTCCAAAAATCTGC; CTGGGTTTTGTTCCCTATTTTTCTCTCCTGTCCTCCCTCACCCCCTCCTTCATATGAAAGGTGCCATGG; AAGAGGCTACAGGGCCAAACGCTGAGCCACCTGCCCTTTTTTCTGCCTCCTTTAGTAAAACTCCGAGTG; AACTGGTCTTCCTTTTTGGTTTTTACTTAACTGTTTCAAAGCCAAGACCTCACACACACAAAAAATGCA; CAAACAATGCAATCAACAGAAAAGCTGTAAATGTGTGTACAGTTGGCATGGTAGTATACAAAAAGATTG; TAGTGGATCTAATTTTTAAGAAATTTTGCCTTTAAGTTATTTTACCTGTTTTTGTTTCTTGTTTTGAAA; GATGCGCATTCTAACCTGGAGGTCAATGTTATGTATTTATTTATTTATTTATTTGGTTCCCTTCCTATT; CCAAGCTTCCATAGCTGCTGCCCTAGTTTTCTTTCCTCCTTTCCTCCTCTGACTTGGGGACCTTTTGGG; GGAGGGCTGCGACGCTTGCTCTGTTTGTGGGGTGACGGGACTCAGGCGGGACAGTGCTGCAGCTCCCTG; GCTTCTGTGGGGCCCCTCACCTACTTACCCAGGTGGGTCCCGGCTCTGTGGGTGATGGGGAGGGGCATT; GCTGACTGTGTATATAGGATAATTATGAAAAGCAGTTCTGGATGGTGTGCCTTCCAGATCCTCTCTGGG; GCTGTGTTTTGAGCAGCAGGTAGCCTGCTGGTTTTATCTGAGTGAAATACTGTACAGGGGAATAAAAGA; GATCTTATTTTTTTTTTTATACTTGGCGTTTTTTGAATAAAAACCTTTTGTCTTAA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002648.4
• Locus ID: 5292
• Summary: The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805, 1825810).[provided by RefSeq, Aug 2011]