CCN2 (NM_001901) Human 3' UTR Clone

SKU SC212423

3' UTR clone of connective tissue growth factor (CTGF) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: CCN2
• Synonyms: CTGF; HCS24; IGFBP8; NOV2
• ACCN: NM_001901
• Insert Size: 1118 bp
• Sequence Data:
  Insert Sequence

  >SC212423 3’UTR clone of NM_001901 ; The sequence shown below is from the reference sequence of NM_001901. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; TACAGGAAGATGTACGGAGACATGGCATGAAGCCAGAGAGTGAGAGACATTAACTCATTAGACTGGAAC; TTGAACTGATTCACATCTCATTTTTCCGTAAAAATGATTTCAGTAGCACAAGTTATTTAAATCTGTTTT; TCTAACTGGGGGAAAAGATTCCCACCCAATTCAAAACATTGTGCCATGTCAAACAAATAGTCTATCAAC; CCCAGACACTGGTTTGAAGAATGTTAAGACTTGACAGTGGAACTACATTAGTACACAGCACCAGAATGT; ATATTAAGGTGTGGCTTTAGGAGCAGTGGGAGGGTACCAGCAGAAAGGTTAGTATCATCAGATAGCATC; TTATACGAGTAATATGCCTGCTATTTGAAGTGTAATTGAGAAGGAAAATTTTAGCGTGCTCACTGACCT; GCCTGTAGCCCCAGTGACAGCTAGGATGTGCATTCTCCAGCCATCAAGAGACTGAGTCAAGTTGTTCCT; TAAGTCAGAACAGCAGACTCAGCTCTGACATTCTGATTCGAATGACACTGTTCAGGAATCGGAATCCTG; TCGATTAGACTGGACAGCTTGTGGCAAGTGAATTTGCCTGTAACAAGCCAGATTTTTTAAAATTTATAT; TGTAAATATTGTGTGTGTGTGTGTGTGTGTATATATATATATATGTACAGTTATCTAAGTTAATTTAAA; GTTGTTTGTGCCTTTTTATTTTTGTTTTTAATGCTTTGATATTTCAATGTTAGCCTCAATTTCTGAACA; CCATAGGTAGAATGTAAAGCTTGTCTGATCGTTCAAAGCATGAAATGGATACTTATATGGAAATTCTGC; TCAGATAGAATGACAGTCCGTCAAAACAGATTGTTTGCAAAGGGGAGGCATCAGTGTCCTTGGCAGGCT; GATTTCTAGGTAGGAAATGTGGTAGCCTCACTTTTAATGAACAAATGGCCTTTATTAAAAACTGAGTGA; CTCTATATAGCTGATCAGTTTTTTCACCTGGAAGCATTTGTTTCTACTTTGATATGACTGTTTTTCGGA; CAGTTTATTTGTTGAGAGTGTGACCAAAAGTTACATGTTTGCACCTTTCTAGTTGAAAATAAAGTGTAT; ATTTTTTCTATAAA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001901.4
• Locus ID: 1490
• Summary: The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq, Nov 2009]