mtTFA (TFAM) (NM_003201) Human 3' UTR Clone

CAT#: SC212176

3' UTR clone of transcription factor A mitochondrial (TFAM) nuclear gene encoding mitochondrial protein for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: mtTFA
• Synonyms: MTDPS15; MTTF1; MTTFA; TCF6; TCF6L1; TCF6L2; TCF6L3
• ACCN: NM_003201
• Insert Size: 1078 bp
• Sequence Data:
  Insert Sequence

  >SC212176 3' UTR clone of NM_003201
  The sequence shown below is from the reference sequence of NM_003201. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TATGGTGCTGAGGAGTGTTAAAAGTAGAAGATTGAGATGTGTTCACAATGGATAGGCACAGGAAACCAGT
  TAGGTCTCAATACCTGAAGCTATCGTAAAATTAAGAAAGGATAAAGTTGGTAAACCTTTTATATTTAGTA
  TCTTTTTATTCAGCTCATGGACTTCTGCCAGCATAATACTTGCTTTGGAAAACCCAGATAAAGGTTCATG
  CAAACTTTATTTTGTGTTTAGGAACTACTGAGGATCAGAGTAATCCAAGCAAATGTGAATCATTTTACCT
  TTGACAAAGGTAAATCAGACTATGAAGTTTTTTTTATACAGGATGATGACTATGGAAAGAGTACTCTTGT
  TTCCTTATATTATGGAGGCAGGAGTTTCGTTTTCAAAATTGTTACAAATTGTAGAAGCCACGGTGTTCTG
  TGATATAAGTGTGTGTTTTTCATAAAGCAGGCAGAACTCATCTAGGTAAATTACAGTTCCTAGGTATAAT
  TCACATTGTATTCAGAGTTGATGGTTGTACATATAAGTGATTGCTGGTTTTAGTTGCAACTTTGTATAAA
  AGGGACTGAGAAATTTATAAACTTTTTTCTTACTGTCTTTTTTCTAAAGTAAAAACAAAGAAATTATGTG
  CCAGATTTATGCATATTATTTTATGTTGCATAGAATAAAATTTTTAATCTTTAATTTTACATTTCCTAAA
  TATATTTTAAGACGAAACATTTGTTCTATAGCTTTTCCCTTTTTTTAAGTAAGGAATTTTATTTTTTTCT
  GAATTATTTTCTCTCGTGAGTATATTGATCCAGAAAGAAAACTTGTATTATGTGTGTTTTAAAATGAGAA
  ATCTAAAAAACGAAAAGTCTCCAAAGTCTCTGGAATTTGAAACACTTTGCATAACGTATAAAAGCCTGTT
  TAAGAGACAGCCAACTATGGCCTGTGGATCAAATCCAGCCTGCTGCCTGCTTTTTATGGCCTGTGAGCTA
  GGAATTGTGTTTATAATTTTAAATGTTTTTTTTTAAAGACTTTTATGATACTTGAAAATTAACATGAATA
  TTTAGTGTTCATAAATAAAGTTTGTTGA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003201.1
• Summary: This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimer's and Parkinson's diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
• Locus ID: 7019