mtTFA (TFAM) (NM_003201) Human 3' UTR Clone

SKU SC212176

3' UTR clone of transcription factor A mitochondrial (TFAM) nuclear gene encoding mitochondrial protein for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: mtTFA
• Synonyms: MTDPS15; MTTF1; MTTFA; TCF6; TCF6L1; TCF6L2; TCF6L3
• ACCN: NM_003201
• Insert Size: 1078 bp
• Sequence Data:
  Insert Sequence

  >SC212176 3' UTR clone of NM_003201; The sequence shown below is from the reference sequence of NM_003201. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon ; ; ; CAATTGGCAGAGCTCAGAATTCAAGCGATCGC; ; TATGGTGCTGAGGAGTGTTAAAAGTAGAAGATTGAGATGTGTTCACAATGGATAGGCACAGGAAACCAGT; TAGGTCTCAATACCTGAAGCTATCGTAAAATTAAGAAAGGATAAAGTTGGTAAACCTTTTATATTTAGTA; TCTTTTTATTCAGCTCATGGACTTCTGCCAGCATAATACTTGCTTTGGAAAACCCAGATAAAGGTTCATG; CAAACTTTATTTTGTGTTTAGGAACTACTGAGGATCAGAGTAATCCAAGCAAATGTGAATCATTTTACCT; TTGACAAAGGTAAATCAGACTATGAAGTTTTTTTTATACAGGATGATGACTATGGAAAGAGTACTCTTGT; TTCCTTATATTATGGAGGCAGGAGTTTCGTTTTCAAAATTGTTACAAATTGTAGAAGCCACGGTGTTCTG; TGATATAAGTGTGTGTTTTTCATAAAGCAGGCAGAACTCATCTAGGTAAATTACAGTTCCTAGGTATAAT; TCACATTGTATTCAGAGTTGATGGTTGTACATATAAGTGATTGCTGGTTTTAGTTGCAACTTTGTATAAA; AGGGACTGAGAAATTTATAAACTTTTTTCTTACTGTCTTTTTTCTAAAGTAAAAACAAAGAAATTATGTG; CCAGATTTATGCATATTATTTTATGTTGCATAGAATAAAATTTTTAATCTTTAATTTTACATTTCCTAAA; TATATTTTAAGACGAAACATTTGTTCTATAGCTTTTCCCTTTTTTTAAGTAAGGAATTTTATTTTTTTCT; GAATTATTTTCTCTCGTGAGTATATTGATCCAGAAAGAAAACTTGTATTATGTGTGTTTTAAAATGAGAA; ATCTAAAAAACGAAAAGTCTCCAAAGTCTCTGGAATTTGAAACACTTTGCATAACGTATAAAAGCCTGTT; TAAGAGACAGCCAACTATGGCCTGTGGATCAAATCCAGCCTGCTGCCTGCTTTTTATGGCCTGTGAGCTA; GGAATTGTGTTTATAATTTTAAATGTTTTTTTTTAAAGACTTTTATGATACTTGAAAATTAACATGAATA; TTTAGTGTTCATAAATAAAGTTTGTTGA; ; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003201.1
• Locus ID: 7019
• Summary: This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimer's and Parkinson's diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]