MSH3 (NM_002439) Human 3' UTR Clone

SKU SC211522

3' UTR clone of mutS homolog 3 (E. coli) (MSH3) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: MSH3
• Synonyms: DUP; FAP4; MRP1
• ACCN: NM_002439
• Insert Size: 983 bp
• Sequence Data:
  Insert Sequence

  >SC211522 3’UTR clone of NM_002439 ; The sequence shown below is from the reference sequence of NM_002439. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; GAAGAAACACAGACTTCTCTTCTTCATTAAAATGAAGACTACATTTGTGAACAAAAAATGGAGAATTAA; AAATACCAACTGTACAAAATAACTCTCCAGTAACAGCCTATCTTTGTGTGACATGTGAGCATAAAATTA; TGACCATGGTATATTCCTATTGGAAACAGAGAGGTTTTTCTGAAGACAGTCTTTTTCAAGTTTCTGTCT; TCCTAACTTTTCTACGTATAAACACTCTTGAATAGACTTCCACTTTGTAATTAGAAAATTTTATGGACA; GTAAGTCCAGTAAAGCCTTAAGTGGCAGAATATAATTCCCAAGCTTTTGGAGGGTGATATAAAAATTTA; CTTGATATTTTTATTTGTTTCAGTTCAGATAATTGGCAACTGGGTGAATCTGGCAGGAATCTATCCATT; GAACTAAAATAATTTTATTATGCAACCAGTTTATCCACCAAGAACATAAGAATTTTTTATAAGTAGAAA; GAATTGGCCAGGCATGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTAGGCAGATCAC; CTGAGGTCAGGAGTTCAAGACCAGCCTGGCCAACATGGCAAAACCCCATCTTTACTAAAAATATAAAGT; ACATCTCTACTAAAAATACGAAAAAATTAGCTGGGCATGGTGGCGCACACCTGTAGTCCCAGCTACTCC; GGAGGCTGAGGCAGGAGAATCTCTTGAACCTGGGAGGCGGAGGTTGCAATGAGCCGAGATCACGTCACT; GCACTCCAGCTTGGGCAACAGAGCAAGACTCCATCTCAAAAAAAAAAAAAGAAAAAAGAAAAGAAATAG; AATTATCAAGCTTTTAAAAACTAGAGCACAGAAGGAATAAGGTCATGAAATTTAAAAGGTTAAATATTG; TCATAGGATTAAGCAGTTTAAAGATTGTTGGATGAAATTATTTGTCATTCATTCAAGTAATAAATATTT; AATGAATACTTGCTATA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002439.5
• Locus ID: 4437
• Summary: The protein encoded by this gene forms a heterodimer with MSH2 to form MutS beta, part of the post-replicative DNA mismatch repair system. MutS beta initiates mismatch repair by binding to a mismatch and then forming a complex with MutL alpha heterodimer. This gene contains a polymorphic 9 bp tandem repeat sequence in the first exon. The repeat is present 6 times in the reference genome sequence and 3-7 repeats have been reported. Defects in this gene are a cause of susceptibility to endometrial cancer. [provided by RefSeq, Mar 2011]