MTOR (NM_004958) Human 3' UTR Clone

SKU SC211383

3' UTR clone of mechanistic target of rapamycin (serine/threonine kinase) (MTOR) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: MTOR
• Synonyms: FRAP; FRAP1; FRAP2; RAFT1; RAPT1; SKS
• ACCN: NM_004958
• Insert Size: 980 bp
• Sequence Data:
  Insert Sequence

  >SC211383 3’UTR clone of NM_004958 ; The sequence shown below is from the reference sequence of NM_004958. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; TGCTATATTGGCTGGTGCCCTTTCTGGTAACTGGAGGCCCAGATGTGCCCATCACGTTTTTTCTGAGGC; TTTTGTACTTTAGTAAATGCTTCCACTAAACTGAAACCATGGTGAGAAAGTTTGACTTTGTTAAATATT; TTGAAATGTAAATGAAAAGAACTACTGTATATTAAAAGTTGGTTTGAACCAACTTTCTAGCTGCTGTTG; AAGAATATATTGTCAGAAACACAAGGCTTGATTTGGTTCCCAGGACAGTGAAACATAGTAATACCACGT; AAATCAAGCCATTCATTTTGGGGAACAGAAGATCCATAACTTTAGAAATACGGGTTTTGACTTAACTCA; CAAGAGAACTCATCATAAGTACTTGCTGATGGAAGAATGACCTAGTTGCTCCTCTCAACATGGGTACAG; CAAACTCAGCACAGCCAAGAAGCCTCAGGTCGTGGAGAACATGGATTAGGATCCTAGACTGTAAAGACA; CAGAAGATGCTGACCTCACCCCTGCCACCTATCCCAAGACCTCACTGGTCTGTGGACAGCAGCAGAAAT; GTTTGCAAGATAGGCCAAAATGAGTACAAAAGGTCTGTCTTCCATCAGACCCAGTGATGCTGCGACTCA; CACGCTTCAATTCAAGACCTGACCGCTAGTAGGGAGGTTTATTCAGATCGCTGGCAGCCTCGGCTGAGC; AGATGCACAGAGGGGATCACTGTGCAGTGGGACCACCCTCACTGGCCTTCTGCAGCAGGGTTCTGGGAT; GTTTTCAGTGGTCAAAATACTCTGTTTAGAGCAAGGGCTCAGAAAACAGAAATACTGTCATGGAGGTGC; TGAACACAGGGAAGGTCTGGTACATATTGGAAATTATGAGCAGAACAAATACTCAACTAAATGCACAAA; GTATAAAGTGTAGCCATGTCTAGACACCATGTTGTATCAGAATAATTTTTGTGCCAATAAATGACATCA; GAATTTTAAACATA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004958.4
• Locus ID: 2475
• Summary: The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This kinase is a component of two distinct complexes, mTORC1, which controls protein synthesis, cell growth and proliferation, and mTORC2, which is a regulator of the actin cytoskeleton, and promotes cell survival and cell cycle progression. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Inhibitors of mTOR are used in organ transplants as immunosuppressants, and are being evaluated for their therapeutic potential in SARS-CoV-2 infections. Mutations in this gene are associated with Smith-Kingsmore syndrome and somatic focal cortical dysplasia type II. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Aug 2020]