Collagen XVII (COL17A1) (NM_000494) Human 3' UTR Clone

CAT#: SC211303

3' UTR clone of collagen type XVII alpha 1 (COL17A1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: COL17A1
• Synonyms: BA16H23.2; BP180; BPA-2; BPAG2; EBR2A; ERED; JEB-I; LAD-1
• ACCN: NM_000494
• Insert Size: 979 bp
• Sequence Data:
  Insert Sequence

  >SC211303 3’UTR clone of NM_000494
  The sequence shown below is from the reference sequence of NM_000494. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  AGGAGAAGAAGTATTGCTGTCAAGCCGTGAGCTAGCCATGGCAGGACAGCTCCTGGACCAGGTCTCATA
  ATGCATGTGGCACTTAGGTCCAAGGTCTCCAGAGGGTGAAAGCTGGAGTCTGTCAATGTCCTACTGAGA
  CAGCACAGCCAACCTAGCTAGCAACATTTGTTTTAGTCTGAACAATATATACTTATAGAATTCAGTCAA
  AGATACACAATCTGAAACAGCTTCATGGGGTGGACTCTAACAGTAGTTGCAATGTTTTAGAATGAGACT
  TACTTCTCTGCTATCTAGATCTGAACTCCTTGGCTTCTTTACTTAGTTCAAGCCCCAGCCTAGGAAAGC
  CAGTTACATAAAAGTTGGCTCAGGAGTCTTAGAGCTTTACCTAAATATGAGCCCAGAAAACGGAGGATG
  GGGGTGGGGCGCCTTCCTGGAGGTGACACTTGATGGGGGTGTGTTCTGGTTACTGTTCTAAGGCTGTGC
  CATCAGCTCCTTCCTCCCCTGTTCATTCTGCATTCTCTAGTCAGTTGGCTAAGAAGTGACTCTTGCAAC
  TAAAAAAATTAAGAAATTCACTTCCCCTCTAGGAGGTGATGATAGGGTTTCTAATGGTTATATGTATAT
  CACATTCCCATTTGCTTAGAAAGTCTGATTGTAGCTATGATTGTCCGTAGGCCCATACTAGAGTTCATG
  GATATGTTATACTGAACCAGGCCAGAGCAAACAGAAAAAGAAGGTTGAGGGCAATGGACAAGGAAGGAA
  TAAAGGGAGAAGAGGGAAAACAGAAAACCTGATGCTGGGGACACAGCATCAGCTCAAGACGTCACCCTC
  CATTCTGCACTCAGAAAATGGCACTTGGGGGACTGGGCGCAGTTGGTCTTTAACCACTTTTCAATGTCT
  AAAAACATTTGTTTGTGGTCTATAAGATGAAACATCATTTCAATCGTAAAATTTCCCATTAAAGAAGTT
  TTTTTTTCTAATA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000494.4
• Summary: This gene encodes the alpha chain of type XVII collagen. Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in this gene are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD-1, is generated by proteolytic processing of the full length form. [provided by RefSeq, Jul 2008]
• Locus ID: 1308
• MW: 38