Collagen XVII (COL17A1) (NM_000494) Human 3' UTR Clone

SKU SC211303

3' UTR clone of collagen type XVII alpha 1 (COL17A1) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: Collagen XVII
• Synonyms: BA16H23.2; BP180; BPA-2; BPAG2; EBR2A; ERED; JEB-I; LAD-1
• ACCN: NM_000494
• Insert Size: 979 bp
• Sequence Data:
  Insert Sequence

  >SC211303 3’UTR clone of NM_000494 ; The sequence shown below is from the reference sequence of NM_000494. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; AGGAGAAGAAGTATTGCTGTCAAGCCGTGAGCTAGCCATGGCAGGACAGCTCCTGGACCAGGTCTCATA; ATGCATGTGGCACTTAGGTCCAAGGTCTCCAGAGGGTGAAAGCTGGAGTCTGTCAATGTCCTACTGAGA; CAGCACAGCCAACCTAGCTAGCAACATTTGTTTTAGTCTGAACAATATATACTTATAGAATTCAGTCAA; AGATACACAATCTGAAACAGCTTCATGGGGTGGACTCTAACAGTAGTTGCAATGTTTTAGAATGAGACT; TACTTCTCTGCTATCTAGATCTGAACTCCTTGGCTTCTTTACTTAGTTCAAGCCCCAGCCTAGGAAAGC; CAGTTACATAAAAGTTGGCTCAGGAGTCTTAGAGCTTTACCTAAATATGAGCCCAGAAAACGGAGGATG; GGGGTGGGGCGCCTTCCTGGAGGTGACACTTGATGGGGGTGTGTTCTGGTTACTGTTCTAAGGCTGTGC; CATCAGCTCCTTCCTCCCCTGTTCATTCTGCATTCTCTAGTCAGTTGGCTAAGAAGTGACTCTTGCAAC; TAAAAAAATTAAGAAATTCACTTCCCCTCTAGGAGGTGATGATAGGGTTTCTAATGGTTATATGTATAT; CACATTCCCATTTGCTTAGAAAGTCTGATTGTAGCTATGATTGTCCGTAGGCCCATACTAGAGTTCATG; GATATGTTATACTGAACCAGGCCAGAGCAAACAGAAAAAGAAGGTTGAGGGCAATGGACAAGGAAGGAA; TAAAGGGAGAAGAGGGAAAACAGAAAACCTGATGCTGGGGACACAGCATCAGCTCAAGACGTCACCCTC; CATTCTGCACTCAGAAAATGGCACTTGGGGGACTGGGCGCAGTTGGTCTTTAACCACTTTTCAATGTCT; AAAAACATTTGTTTGTGGTCTATAAGATGAAACATCATTTCAATCGTAAAATTTCCCATTAAAGAAGTT; TTTTTTTCTAATA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000494.4
• Locus ID: 1308
• Summary: This gene encodes the alpha chain of type XVII collagen. Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in this gene are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD-1, is generated by proteolytic processing of the full length form. [provided by RefSeq, Jul 2008]