Vesicle docking protein p115 (USO1) (NM_003715) Human 3' UTR Clone

SKU SC211220

3' UTR clone of USO1 homolog vesicle docking protein (yeast) (USO1) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: Vesicle docking protein p115
• Synonyms: P115; TAP; VDP
• ACCN: NM_003715
• Insert Size: 1021 bp
• Sequence Data:
  Insert Sequence

  >SC211220 3’UTR clone of NM_003715 ; The sequence shown below is from the reference sequence of NM_003715. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; GATCCTGGCAAGGATCTAGATCATATCTAGTTTTCAAATCTCTAGGAACAATAGAGATTATATCATAAC; TCTGAAGATGGACTCTAAACTTTGGTTTACCTCCATGGAAAATAAAGATTTAGAAACCAGGTGGAAAAC; ATTCACTATTAAGACTATTGATATATTTTTGTAATGTTGCCACCCATGTTGAAAACCTTAAAACTGAAT; TTATGTAGAACACACATCTTTTATTTAAATCCATCTGAACTGTCCCAAAATGTAATTTGCAAAGAGCTT; CAAACACCAAAAATATACCATTTTAAGGCATGTGGTGACAATGCTATTTGCCTTAGTTCATTCGTTGTT; TACTTTGCAAAATTTGTGACTTTTAAGTACTAACACATTTGATTGAATGTGTATTGAATTGGAATTATT; ATTTCTAAATTCAGCATTTGAGTCCTTAGTTGATGATTTTCTTTTAAAGAAATTAGTTTATTTAAAAGC; AAGATAGCCTGGGTTGGGAGTTAGTGCATATCATCAGCCCTCCATCTAATGTGATTGCTAAGATGTGCA; GGATAGGACAGCTTAGAATGAGTGCCTTGACACCTACATATGGCTGCATTCCATACCTCAGTGAAGAAG; CCGAAGGATGCTGACACACAGTGATGTGGAGGGCATTCCGGAGAAACTTTTGCAACAGTGTATTAATGT; GATTGGTTTTCTTTTTGTTTTAAGCTAAACATCTAAAACAAATTTCAAGTTAACAGTTCATACAACTCA; GATTTAGTAGTATTGTTTATCATAAAACATATTATGTTCCTAGTTTTAAAAAGACCCAAAGTATGATTA; ATATAATTTATTGGCATTTTTGCTGAATAGGTTTAAGTCAGATAATAGATTTTAAAAAAGCAAATGAGG; CAATGTGTCAAAATTTAATGTTTCATAATAAAAATAGATACGTTGACTTAATCCTTGGTCATGGTTCCC; TTTGTTTACATCTTAAAAGTATTTTATTCTTAATAAAAGATGAGGCAAGTGAAAA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003715.4
• Locus ID: 8615
• Summary: The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]