GPNMB (NM_002510) Human 3' UTR Clone

SKU SC210907

3' UTR clone of glycoprotein (transmembrane) nmb (GPNMB) transcript variant 2 for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: GPNMB
• Synonyms: HGFIN; NMB; PLCA3
• ACCN: NM_002510
• Insert Size: 914 bp
• Sequence Data:
  Insert Sequence

  >SC210907 3’UTR clone of NM_002510 ; The sequence shown below is from the reference sequence of NM_002510. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; AAAAACCAAGAATTTAAAGGAGTTTCTTAAATTTCGACCTTGTTTCTGAAGCTCACTTTTCAGTGCCAT; TGATGTGAGATGTGCTGGAGTGGCTATTAACCTTTTTTTCCTAAAGATTATTGTTAAATAGATATTGTG; GTTTGGGGAAGTTGAATTTTTTATAGGTTAAATGTCATTTTAGAGATGGGGAGAGGGATTATACTGCAG; GCAGCTTCAGCCATGTTGTGAAACTGATAAAAGCAACTTAGCAAGGCTTCTTTTCATTATTTTTTATGT; TTCACTTATAAAGTCTTAGGTAACTAGTAGGATAGAAACACTGTGTCCCGAGAGTAAGGAGAGAAGCTA; CTATTGATTAGAGCCTAACCCAGGTTAACTGCAAGAAGAGGCGGGATACTTTCAGCTTTCCATGTAACT; GTATGCATAAAGCCAATGTAGTCCAGTTTCTAAGATCATGTTCCAAGCTAACTGAATCCCACTTCAATA; CACACTCATGAACTCCTGATGGAACAATAACAGGCCCAAGCCTGTGGTATGATGTGCACACTTGCTAGA; CTCAGAAAAAATACTACTCTCATAAATGGGTGGGAGTATTTTGGTGACAACCTACTTTGCTTGGCTGAG; TGAAGGAATGATATTCATATATTCATTTATTCCATGGACATTTAGTTAGTGCTTTTTATATACCAGGCA; TGATGCTGAGTGACACTCTTGTGTATATTTCCAAATTTTTGTACAGTCGCTGCACATATTTGAAATCAT; ATATTAAGACTTTCCAAAGATGAGGTCCCTGGTTTTTCATGGCAACTTGATCAGTAAGGATTTCACCTC; TGTTTGTAACTAAAACCATCTACTATATGTTAGACATGACATTCTTTTTCTCTCCTTCCTGAAAAATAA; AGTGTGGGAAGAGACAA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002510.3
• Locus ID: 10457
• Summary: The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]