FAT (FAT1) (NM_005245) Human 3' UTR Clone

SKU SC210146

3' UTR clone of FAT tumor suppressor homolog 1 (Drosophila) (FAT1) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: FAT
• Synonyms: CDHF7; CDHR8; FAT; hFat1; ME5
• ACCN: NM_005245
• Insert Size: 828 bp
• Sequence Data:
  Insert Sequence

  >SC210146 3’UTR clone of NM_005245 ; The sequence shown below is from the reference sequence of NM_005245. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; CTGGATTCCCAGCAGCACACGGAAGTCTGACTCTCAACTCCCCCCAAAGTGCCTGACTTTAGTGAACCT; AGAGGTGATGTGAGTAATCCGCGCTGTTCTTTGCAGCAGTGCTTCCAAGCTTTTTTTGGTGAGCCGAAT; GGGCATGGCTGCGCTGGATCCTGCGCCTCTGGACGTGCTAGCCATTTCCAGTGTCCCAACTACTGTCAT; CGTGAGGTTTTCATCGGCTGTGCCATTTCCCAACGTCTTTTGGGATTTACATCTGTCTGTGTTAAAATA; ATCAAACGAAAAATCAGTCCTGTGTTGTCAGCATGATTCATGTATTTATATAGATTTGATTATTTTAAT; TTTCCTGTCTCTTTTTTTTGTAAATTTTATGTACAGATTTGATTTTTCATAGTTTTAACTAGATTTCCA; AGATATTTTGTGCATTTGTTTCAACTGAATTTTGGTGGTGTCAGTGCCATTATCTAGCACCCTGATTTT; TTTTTTTTTACTATAACCAGGGTTTCATTCTGTCTTTTTCCACTGAAGTGTGACATTTTGTTAGTACAT; TTCAGTGTAGTCATTCATTTCTAGCTGTACATAGGATGAAGGAGAGATCAGATACATGAACATGTCTTA; CATGGGTTGCTGTATTTAGAATTATAAACATTTTTCATTATTGGAAAGTGTAACGGGGACCTTCTGCAT; ACCTGTTTAGAACCAAAACCACCATGACACAGTTTTTATAGTGTCTGTATATTTGTGATGCAATGGTCT; TGTAAAGGTTTTTAATGAAAACTACCATTAGCCAGTCTTTCTTACTGACAATAAATTATTAATAAAATA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005245.4
• Locus ID: 2195
• Summary: This gene is an ortholog of the Drosophila fat gene, which encodes a tumor suppressor essential for controlling cell proliferation during Drosophila development. The gene product is a member of the cadherin superfamily, a group of integral membrane proteins characterized by the presence of cadherin-type repeats. In addition to containing 34 tandem cadherin-type repeats, the gene product has five epidermal growth factor (EGF)-like repeats and one laminin A-G domain. This gene is expressed at high levels in a number of fetal epithelia. Its product probably functions as an adhesion molecule and/or signaling receptor, and is likely to be important in developmental processes and cell communication. Transcript variants derived from alternative splicing and/or alternative promoter usage exist, but they have not been fully described. [provided by RefSeq, Jul 2008]