Axin 1 (AXIN1) (NM_003502) Human 3' UTR Clone

SKU SC209177

3' UTR clone of axin 1 (AXIN1) transcript variant 1 for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: Axin 1
• Synonyms: AXIN; PPP1R49
• ACCN: NM_003502
• Insert Size: 712 bp
• Sequence Data:
  Insert Sequence

  >SC209177 3’UTR clone of NM_003502 ; The sequence shown below is from the reference sequence of NM_003502. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; ATCATCGGCAAAGTGGAGAAGGTGGACTGATAGGCTGGTGGGCTGGCCGCTGTGCCAGGCGAGGCCCTT; GGCGGGCACGGGTGTCACGGCCAGGCAGATGACCTCGTACTCAGGAGCCCGATGGGGAACAGTGTTGGG; TGTACCACCCATCCCTGTGGTCTACCCGTGTCTAGAGGCAGGTAGGGGGTCCCTCCAAGTGGTCCACAA; GCTTCTGTCCTGCCCCCAAGGAGGCAGCCTGGACCACTCCTCATAGCAATACTTGGAGGGCCCAGCCCA; AGTGAGGCAGCCGAGGTCCCTGCTGCCAGCTTCAGGTGACCCCCCCCCATCCCCCGGCACCTCCCTTGG; GCACGTGTGCTGGGATCTACTTTCCCTCTGGGATTTGCCCACGTACCCAGGTCTGGGTGGGGCCCAGGC; CCGGATGCAGAGGCCTGCAGGGCCTCTGTCAATTGTACGCGCCACCGAGTGCCTTCAACACAGCTTGTC; TCTTGCCTGCCACTGTGTGAATCGGCGACGGAGCACTGCACCTGCCTCCAGCCGCCGGCTGTGCAGTCC; TGGGTCCTCCTTTCTGAGGGCCCGTGTAAATATGTACATTTCTCAGGCTAGGCCAGCAGGGGCTGCCCG; AGTCTGTTTTTCATGCGATGACACTTGTACAATTAATTATCTTTTCAAAGGTACTTGGATAATAATGAA; ATAAAACTGTTTTTGAACCTGC; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003502.4
• Locus ID: 8312
• Summary: This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]