PION (GSAP) (NM_017439) Human 3' UTR Clone

SKU SC208212

3' UTR clone of pigeon homolog (Drosophila) (PION) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: PION
• Synonyms: PION
• ACCN: NM_017439
• Insert Size: 637 bp
• Sequence Data:
  Insert Sequence

  >SC208212 3’UTR clone of NM_017439 ; The sequence shown below is from the reference sequence of NM_017439. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; AAACACACCGCGATGCTTTTAGGCTTATGAAAAAGAAAACGCAATTGGATCTGCTGCTGCCATTTTAAT; CTTGCTCATTAACCTTACTCCTTTGAGAATTCTTTAACAATATTTAAAATTGGTAACAAAAATAGTTTA; GCCATAATTGTTTAGCCATGTGAGTTTCAGGTTGGTACACGTTCAGACAGAACTGCTGTATCACATTCC; AATTTTGAATAGCCAGTGAGCAATCAAGTGTAGAGAAATGATAAATGGCCTAAGAAGGCATACAGTGGC; ATAAACGATGCTCTTCCTAGTAGCTTAATAGGCCACAAGCTAGTTTCTGTTGCACTCTGAAATAAAATA; TGCTTTAAAAATGTAGGGAACAGTGCTTAGAAAAGCAAAAACTAGGTGTGTCATTGAAATAATAGGCAT; AAAAATTAAATGTTACATAAGAACACTATTTGGAAAGAGGGTCCTTTTAAAAACTGAATTTGTACTAAA; TCAGATTTGCCATGTCCAGTACAGAATAATTTGTACTTAGTATTTGCAGCAGGGTTTGTCTTTGTGAAT; TCAGATGAAACATATTTATTTTTTTTTATTTATAAAAGGTTGATTTAGGAATATTTTGTCAGTCATTAA; AAAACCTGAAAACATA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_017439.4
• Locus ID: 54103
• Summary: Accumulation of neurotoxic amyloid-beta is a major hallmark of Alzheimer disease (AD; MIM 104300). Formation of amyloid-beta is catalyzed by gamma-secretase (see PSEN1; MIM 104311), a protease with numerous substrates. PION, or GSAP, selectively increases amyloid-beta production through a mechanism involving its interaction with both gamma-secretase and its substrate, the amyloid-beta precursor protein (APP; MIM 104760) C-terminal fragment (APP-CTF) (He et al., 2010 [PubMed 20811458]).[supplied by OMIM, Nov 2010]