PPP1R14B (NM_138689) Human 3' UTR Clone

SKU SC204307

3' UTR clone of protein phosphatase 1 regulatory (inhibitor) subunit 14B (PPP1R14B) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: PPP1R14B
• Synonyms: PHI-1; PLCB3N; PNG; SOM172
• ACCN: NM_138689
• Insert Size: 304 bp
• Sequence Data:
  Insert Sequence

  >SC204307 3' UTR clone of NM_138689; The sequence shown below is from the reference sequence of NM_138689. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon ; ; ; CAATTGGCAGAGCTCAGAATTCAAGCGATCGC; ; CATGCAGAAGCTGAGCACACCCCAGAAGAAGTGACGGTCCCCGACCCAGGAGAACGGTGGCTCCCACAGG; ACAATCGCTGCCCCCCAACCTCGTAGCAACAGCAATACCGGGGGACCCTGCGGCCAGGCCTGGTGCCATG; AGCAGGGCTCCTCGTGCCCCTGGCCCAGGGGTCTCTTCCCCTGCCCCCTCAGTTTTCCACTTTTGGGGTT; TTTTATTGTTATTAAACTGATGGGACTTTTTGTGTTTTTATATTGACTCTGCGGCGCGGGCCCTTTAATA; AAGCTAGGATACGCCTTTGGTGCA; ; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_138689.2
• Locus ID: 26472
• Summary: Inhibitor of PPP1CA. Has over 50-fold higher inhibitory activity when phosphorylated (By similarity).[UniProtKB/Swiss-Prot Function]