PSMA (FOLH1) (NM_001014986) Human 3' UTR Clone

SKU SC201230

3' UTR clone of folate hydrolase (prostate-specific membrane antigen) 1 (FOLH1) transcript variant 2 for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: PSMA
• Synonyms: FGCP; FOLH; GCP2; GCPII; mGCP; NAALAD1; NAALAdase; PSM; PSMA
• ACCN: NM_001014986
• Insert Size: 1694 bp
• Sequence Data:
  Insert Sequence

  >SC201230 3’UTR clone of NM_001014986 ; The sequence shown below is from the reference sequence of NM_001014986. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; GCTGCAGAGACTTTGAGTGAAGTAGCCTAAGAGGATTCTTTAGAGAATCCGTATTGAATTTGTGTGGTA; TGTCACTCAGAAAGAATCGTAATGGGTATATTGATAAATTTTAAAATTGGTATATTTGAAATAAAGTTG; AATATTATATATAGTTATGTGAGTGTTTATATATGTGTGTGTTTATATTGTTTATCTTCTCCCTATGGA; TTAAAACTGAATTTCATAATTATAAGAGGTTATTCTGAAGTGGAAAAATTTAACTCAGTATTAAATCTA; AGGAGAATGGCCTAATATAGTAAAACTCTCATCTGGCATTATCAGGGAATCAAGTCTAATCTATTCATG; TCACTTCACACAGAAGAAAACATCAGTATGTCAGAGAGCACACTGGGGAATATGCACAAGATTATCCCA; AGCCAGAGGCCTCACGGCCTACCTGGCCAGCCTGGGCTGAGAGGATCACTATCTCAGCACACTATTTGG; GAAATGGATCAAATCACACTTTTAGTAAATGTTATCACTCTATAGCATAAGAAATAATTATTTTTTATT; TATATAAAAGGCTATAGTATAAAATATATGTATAGTAATTAAATGAACACTTGTGAACCTAATAGCCAT; ATGAAGAAAATAACATTTCTAATATCTTTGGATGCCCCATGTACTAATGACAGTTATGCTTTTGCATTT; TCTTGAATTTTATGTTTATTTATCTTTCCTCTGTCATTATTTATAATTTTATCACACATGGCTGTATCC; TTTACATGTTTTGGCATTATGTATTTTTGAACTTTTTGTAAAGACAATCATACCATGTGTAATTTTCAG; GGACTTGATTTTTTTCATTGACTTTTAAGGGTTCAAATATATTATCACTGTGGCTGTAGTTTGCCATAT; TTTGCTGATATAGAGCATTCATTCACATGAGGGTAGGATTCAGGGTCCATCAAGACAGAGAAAACATAC; AGTAATGTGAATAGGGAAAGTTAATATGAAGAATTATTAATTGTTACAGCATTGGAACAATGAAATATT; GTCTAGTAATATGTAAAGAGAAGTCTCAAGAATATGTGATGAGCAGATGTAAGGAATTGCTCTTGTCTC; CATGGTGAATTTGGAGCAGCCAATGAAGAGTCCCCTCACATTGTGGCCTCGCTCAAAGTTAAGAAGTCG; CTGTAGTGTTGCCCTTGAAGAATCTGCTTCAAATTGACACTTCAGAACTCCCCAGAAACTTGTCTTCTG; GGCCAATGTGTAAAGCTGTTTATGAAGAAATGTCAAGCCAGAGGGGCTCTACTACAAATTTGGCAAAGG; ACAATTTCAGGAGAAGCTCTTGGCCGCTGGGTTCTCCTGGCCACCATGAACTTCAGGAAGTGGGTGCCA; TAGCAGCAGCCTGAACTACAGAATCTGGGCACTGGTGTAGCTCTGTATGCCCTCCGTGTCAGATGCTGG; AGATGTCATTTGCATTGCCAGAGTTTGCCAAGGGTGCACACAGAAAGCAGATTGAAAAGCACCCTCTTG; GAACATCTCTCCAATGCCTTCTACTCACAAAGTTTAACATCATTAACACGTGACAAAGAAGAACTATTT; AATGGGCCCAGATCTATTTATGAAGACAATCAAGTGGGAGTTTGGAGTGGATAACCCAAATTTGGATAA; CTGGTGAATAATAAAATGTATTTATTTCTGCTGGTGTA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001014986.3
• Locus ID: 2346
• Summary: This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants encoding several different isoforms. [provided by RefSeq, Jul 2010]