Clone OTI2B5, Anti-His mouse monoclonal antibody

CAT#: TA150088

Product Datasheet for 'TA150088'

Cited in 15 publications.

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Product Data
Product Name	Clone OTI2B5, Anti-His mouse monoclonal antibody
Clone Name	OTI2B5 (formerly 2B5)
Applications	WB, Assay
Recommend Dilution	WB: 1:4000
Target	His tag
Host	Mouse
Clonality	Monoclonal
Immunogen	Anti-His monoclonal antibody is produced by immunizing mice with a synthetic peptide (HHHHHH) coupled to KLH
Isotype	IgG1
Formulation	400ug lyophilized powder. The lyophilized antibody can be reconstituted with 100ul of ddH2O. The concentration after reconstitution will be 4 ug/µl.
Concentration	4 mg/ml
Storage Condition	Stable for 1 year at -20ºC from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. After reconstitution, aliquot into small quantity for long term storage at -20ºC. Avoid repeated freezing and thawing. Aliquots for short-term usage will be stable at 4ºC for 3 months.
Synonyms	ANTI HIS, ANTI HISTIDINE, ANTIHIS, anti-His, his tag antibody, Anti-His Antibody, his antibody, anti his tag, anti-his tag

Citations (15)

The use of this Antibodies has been cited in the following citations:
Induction of immune responses by a novel recombinant fusion protein of enterovirus A71 in BALB/c mice ,Issaro, N;Wu, F;Weng, L;Zhou, M;Fang, Z;Huang, S;Rajamanickam, V;Liu, M;Tian, H;Li, X;Jiang, C;, Mol. Immunol. 2018 ,PubMed ID 30465931 [HIS]
Secretory overproduction of a raw starch-degrading glucoamylase in Penicillium oxalicum using strong promoter and signal peptide ,Wang, L;Zhao, S;Chen, XX;Deng, QP;Li, CX;Feng, JX;, Appl. Microbiol. Biotechnol. 2018 ,PubMed ID 30155751 [HIS]
The proinflammatory protein HMGB1 is a substrate of transglutaminase-2 and forms high-molecular weight complexes with autoantigens ,Willis, WL;Wang, L;Wada, TT;Gardner, M;Abdouni, O;Hampton, J;Valiente, G;Young, N;Ardoin, S;Agarwal, S;Freitas, MA;Wu, LC;Jarjour, WN;, J. Biol. Chem. 2018 ,PubMed ID 29618516 [HIS]
LRP6 promotes invasion and metastasis of colorectal cancer through cytoskeleton dynamics ,Yao, Q;An, Y;Hou, W;Cao, YN;Yao, MF;Ma, NN;Hou, L;Zhang, H;Liu, HJ;Zhang, B;, Oncotarget 2017 ,PubMed ID 29312635 [Tag Antibodies]
Rtt101-Mms1-Mms22 coordinates replication-coupled sister chromatid cohesion and nucleosome assembly ,Zhang, J;Shi, D;Li, X;Ding, L;Tang, J;Liu, C;Shirahige, K;Cao, Q;Lou, H;, EMBO Rep. 2017 ,PubMed ID 28615292 [HIS]
Control of Pathological Cardiac Hypertrophy by Transcriptional Corepressor IRF2BP2 (Interferon Regulatory Factor-2 Binding Protein 2) ,Fang, J;Li, T;Zhu, X;Deng, KQ;Ji, YX;Fang, C;Zhang, XJ;Guo, JH;Zhang, P;Li, H;Wei, X;, Hypertension 2017 ,PubMed ID 28716987 [HIS]
A type Ⅲ effector NleF from EHEC inhibits epithelial inflammatory cell death by targeting caspase-4 ,Song, T;Li, K;Zhou, W;Ren, H;Zhou, J;Jin, Y;Dai, H;Xu, T;Hu, M;Yue, J;Liang, L;, BioMed Research International 2017 [HIS]
IDN2 Interacts with RPA and Facilitates DNA Double-Strand Break Repair by Homologous Recombination in Arabidopsis ,Liu, M;Ba, Z;Costa-Nunes, P;Wei, W;Li, L;Kong, F;Li, Y;Chai, J;Pontes, O;Qi, Y;, Plant Cell 2017 ,PubMed ID 28223440 [HIS]
Saposin-like Proteins, a Multigene Family of Schistosoma Species, Are Biomarkers for the Immunodiagnosis of Schistosomiasis Japonica ,Liu, S;Zhou, X;Piao, X;Hou, N;Shen, Y;Zou, Y;Li, S;Cao, J;Chen, Q;, J. Infect. Dis. May 2016 ,PubMed ID 27190177 [His]
Antimicrobial activity of recombinant mature bovine neutrophil ß-defensin 4 on mycobacterial infection ,Kang, JJ;Lyu, Y;Zhao, DM;Tian, LH;Yin, XM;Yang, LF;Teng, KD;Zhou, XM;, Int. J. Tuberc. Lung Dis. Jun 2015 ,PubMed ID 25946365 [His]
Studies on the promiscuous xenobiotic receptor (PXR, NR1I2) in zebrafish: antibody characterisation and genetic variation in zebrafish embryos ,Hitchcock, DJ;, Thesis Jun 2014 [HIS] [WB]
In situ protein microarrays capable of real-time kinetics analysis based on surface plasmon resonance imaging ,Nand, A;Singh, V;Pérez, JB;Tyagi, D;Cheng, Z;Zhu, J;, Anal. Biochem. Jun 2014 ,PubMed ID 24953011 [His] [Assay]
Antimycobacterial activity of Pichia pastoris-derived mature bovine neutrophil ß-defensins 5 ,Kang, J;Zhao, D;Lyu, Y;Tian, L;Yin, X;Yang, L;Teng, K;Zhou, X;, Eur. J. Clin. Microbiol. Infect. Dis. May 2014 ,PubMed ID 24839955 [His] [WB]
Molecular characterization and expression analysis of a GTP-binding protein (MiRab5) in Mangifera indica ,Liu, ZL;Luo, C;Dong, L;Van Toan, C;Wei, PX;He, XH;, Gene, Feb 2014. ,PubMed ID 24560931 [anti-HIS] [WB]
Real-Time Imaging of Notch Activation with a Luciferase Complementation-Based Reporter ,Ma. Xenia G. Ilagan, Sora Lim, Mary Fulbright, David Piwnica-Worms, and Raphael Kopan, Sci. Signal., Jul 2011; 4: rs7. ,PubMed ID 21775282 [His] [WB]

Documents

Product Manuals
Development Guilde Goat anti-Mouse IgG [H&L] Western Blotting Luminol Kit
FAQs
Antibody and Western Standard
SDS
Antibody SDS

Resources

Antibody Resources
Product Overview Video: UltraMAB Product Overview Video: TrueMAB Webinar Recording: UltraMAB Video Tutorial: Video Tutorial: Antibody specificity and Knockout(KO) lysates Western Blot Protocol IHC Protocol IF Protocol FACS Protocol Immunoprecipitation (IP) Protocol IP protocol with magnetic beads

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