Rabbit IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

SKU TA397954

Rabbit IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Product Data

• Application: ELISA, IHC, WB
• Recommended Dilution: WB: 1:1,000 - 1:10,000; IHC: 1:500 - 1:2,500; ELISA: 1:10,000 - 1:50,000
• Antibody Host: Mouse
• Clonality: Polyclonal
• Immunogen: Rabbit IgG whole molecule
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Mouse Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against or Goat, Human and Mouse Serum Proteins.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Reconstitution Method: Restore with deionized water (or equivalent) - Reconstitution Volume: 1.0 mL
• Concentration: 1.0 mg/mL - lot specific
• Conjugation: HRP
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Shipping: Ambient
• Note: Mouse Anti-Rabbit IgG Peroxidase Conjugate has been tested by ELISA and western blot and is assayed against 1.0 µg of Rabbit IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:8,000 to 1:32,000 of the reconstitution concentration is suggested for this product.