Rabbit IgG (H&L) Antibody Pre-adsorbed
| Product Data | |
| Application | ELISA, IHC, WB |
|---|---|
| Recommended Dilution | WB: 1:3,000 - 1:30,000 IHC: 1:1,000 - 1:5,000 ELISA: 1:65,000 |
| Reactivity | Rabbit |
| Antibody Host | Mouse |
| Clonality | Polyclonal |
| Immunogen | Anti-Rabbit IgG (H&L) was produced by repeated immunization with rabbit whole IgG molecule in mouse. |
| Specificity | This product was prepared from monospecific polyclonal ascites by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Goat, Human or Mouse Serum Proteins. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.1 mg/mL - lot specific |
| Conjugation | Unconjugated |
| Storage | Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
| Shipping | Ambient |
| Note | Anti-Rabbit IgG (H&L) has been tested by ELISA and western blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. |
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