Rabbit IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed
| Product Data | |
| Application | ELISA, IHC, WB |
|---|---|
| Recommended Dilution | WB: 1:500 - 1:2,500 IHC: 1:200 - 1:1,000 ELISA: 1:2,000 - 1:10,000 |
| Antibody Host | Guinea Pig |
| Clonality | Polyclonal |
| Immunogen | Rabbit IgG whole molecule |
| Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Guinea Pig Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Goat, Human and Mouse Serum Proteins. |
| Buffer | 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 |
| Concentration | 1.0 mg/mL - lot specific |
| Conjugation | Alkaline Phosphatase |
| Storage | Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
| Shipping | Ambient |
| Note | This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,500 to 1:6,000 is suggested for this product. |
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