Rabbit IgG (H&L) Antibody ATTO 594 Conjugated Pre-Adsorbed

SKU TA397941

Rabbit IgG (H&L) Antibody ATTO 594 Conjugated Pre-Adsorbed

Product Data

• Application: IF, WB
• Recommended Dilution: WB: >1:10,000; IF: >1:5,000; FLISA: >1:20,000
• Antibody Host: Goat
• Clonality: Polyclonal
• Immunogen: Rabbit IgG whole molecule
• Specificity: Rabbit IgG (H&L) Antibody ATTO 594 was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of rabbit IgG and with light chains of most rabbit immunoglobulins.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Reconstitution Method: Restore with deionized water (or equivalent) - Reconstitution Volume: 500 µL
• Concentration: 1.0 mg/mL - lot specific
• Conjugation: ATTO 594
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Shipping: Ambient
• Note: Anti-Rabbit IgG (H&L) conjugated to ATTO 594 has been tested by dot blot and western blot and is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.