Mouse IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-adsorbed
| Product Data | |
| Application | ELISA, IHC, WB |
|---|---|
| Recommended Dilution | WB: 1:2,000 - 1:10,000 IHC: User Optimized ELISA: 1:100,000 |
| Reactivity | Mouse |
| Antibody Host | Goat |
| Clonality | Polyclonal |
| Immunogen | Mouse IgG whole molecule |
| Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Human Serum Proteins. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 500 µL |
| Concentration | 1.0 mg/mL - lot specific |
| Conjugation | HRP |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Shipping | Ambient |
| Note | Mouse secondary antibody conjugated to horseradish peroxidase is available in a variety of formats. Anti-Mouse IgG Peroxidase Antibody has been tested by ELISA and is suitable for ELISA, Immunohistochemistry, western blotting, as well as other peroxidase antibody based assays. |
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