Human IgG F(c) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

SKU TA397723

Human IgG F(c) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

Product Data

• Application: ELISA, IHC, WB
• Recommended Dilution: WB: 1:500 - 1:2,000; IHC: 1:200 - 1:1,000; ELISA: 1:1,000 - 1:5,000
• Antibody Host: Goat
• Clonality: Polyclonal
• Immunogen: Human IgG F(c) fragment
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against Human IgG F(ab) or Mouse Serum Proteins. Specificity was confirmed by ELISA minimal cross reactivity against Mouse IgG.
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
• Concentration: 1.0 mg/mL - lot specific
• Conjugation: Alkaline Phosphatase
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Shipping: Ambient
• Note: Anti-Human IgG F(c) Alkaline Phosphatase Conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.