Bovine IgG (H&L) Antibody Peroxidase Conjugated

SKU TA397122

Bovine IgG (H&L) Antibody Peroxidase Conjugated

Product Data

• Application: ELISA, IHC, WB
• Recommended Dilution: WB: 1:1,000 - 1:10,000; IHC: 1:500 - 1:2,500; ELISA: 1:10,000 - 1:50,000
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Bovine IgG whole molecule
• Specificity: Anti-BOVINE IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Bovine IgG and Bovine Serum.
• Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Reconstitution Method: Restore with deionized water (or equivalent) - Reconstitution Volume: 2.0 mL
• Concentration: 10.0 mg/mL - lot specific
• Conjugation: HRP
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Shipping: Ambient
• Note: Anti-BOVINE IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:17,000 to 1:70,000 of the reconstitution concentration is suggested for this product.