Bovine IgG (H&L) Secondary Antibody

SKU TA397106

Bovine IgG (H&L) Secondary Antibody

Product Data

• Application: ELISA, IHC, WB
• Recommended Dilution: WB: 1:2,000 - 1:10,000; IHC: 1:1,000 - 1:5,000; ELISA: 1:20,000 - 1:100,000
• Antibody Host: Goat
• Clonality: Polyclonal
• Immunogen: Bovine IgG whole molecule
• Specificity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Bovine IgG and Bovine Serum.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Reconstitution Method: Restore with deionized water (or equivalent) - Reconstitution Volume: 5.0 mL
• Concentration: 10.0 mg/mL - lot specific
• Conjugation: Unconjugated
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Shipping: Ambient
• Note: Anti-Bovine IgG Secondary Antibodies have been tested by dot blot and are designed for Western Blotting, ELISA and Immunohistochemistry. Secondary antibodies can also be used for a variety of other applications such as Assay Development, Flow Cytometry and in situ hybridization.