Mouse IgG + IgM (H+L chain), adsorbed Goat Polyclonal Antibody

SKU R1469AP

Mouse IgG + IgM (H+L chain), adsorbed goat polyclonal antibody, AP

Product Data

• Recommended Dilution: Suitable for Immunoblotting (Western or dot blot, 1/500-1/2,500), ELISA (1/3,000-1/12,000) and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
• Reactivity: Mouse
• Antibody Host: Goat
• Clonality: Polyclonal
• Immunogen: Mouse IgG and IgM whole molecules
• Specificity: This product was prepared from polyspecific antiserum by Immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.; Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Alkaline Phosphatase, Mouse IgG and Mouse IgM. No reaction was observed against bovine, horse or human serum proteins.
• Buffer: 0.05 M Tris Chloride, 0.15 M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001 M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.; Label: AP; State: Liquid (sterile filtered) purified IgG fraction.; Label: Alkaline Phosphatase (Calf Intestine) (MW 140,000 daltons)
• Concentration: lot specific
• Purification: Immunoaffinity chromatography.
• Conjugation: AP
• Storage: Store vial at 2-8°C before opening.; DO NOT FREEZE!.; This product is stable at 2-8°C as an undiluted liquid. Dilute only prior to immediate use.; Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.; Avoid repeated freezing and thawing.
• Shipping: Blue Ice