Sheep IgG (H+L chain) Rabbit Polyclonal Antibody

SKU R1456F

Sheep IgG (H+L chain) rabbit polyclonal antibody, FITC

Product Data

• Recommended Dilution: Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.; Recommended Dilutions: ; FLISA: 1/10,000-1/50,000. ; IF Microscopy: 1/1,000-1/5,000. ; Flow Cytometry: 1/500-1/2,500.
• Reactivity: Sheep
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Sheep IgG whole molecule.
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity Chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.; Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Sheep IgG and Sheep Serum.
• Buffer: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2; Label: FITC; State: Lyophilized purified IgG fraction; Stabilizer: 10 mg/ml BSA (IgG and Protease free); Preservative: 0.01% (w/v) Sodium Azide; Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons); Absorption emission: 495 nm / 528 nm; Molar radio: 3.3 moles FITC per mole of Rabbit IgG
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Conjugation: FITC
• Storage: Store vial at 2-8°C prior to restoration. For extended storage mix with glycerol to 50% and then aliquot contents and freeze at -20°C or below. ; Centrifuge product if not completely clear after standing at room temperature.; This product is stable for one month at 2-8°C as an undiluted liquid. ; Dilute only prior to immediate use. ; Avoid repeated freezing and thawing.
• Shipping: Ambient