Sheep IgG (H+L chain) Rabbit Polyclonal Antibody

SKU R1456B

Sheep IgG (H+L chain) rabbit polyclonal antibody, Biotin

Product Data

• Recommended Dilution: Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.; This product has been assayed against 1.0 ug of Sheep IgG in a standard capture ELISA using peroxidase conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6- sulfonic acid]) as a substrate for 30 minutes at room temperature.; A working dilution of 1:20,000 to 1:80,000 is suggested for this product.
• Reactivity: Sheep
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Sheep IgG whole molecule
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Sheep IgG, and Sheep Serum.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% Sodium Azide as preservative; Label: Biotin; State: Lyophilized purified IgG fraction; Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC); Molar radio: 10-20 BAC molecules per Rabbit IgG molecule
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
• Conjugation: Biotin
• Storage: Store vial at 2-8°C prior to restoration. Restore with deionized water. Centrifuge product if not completely clear after standing at room temperature.; This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below.; Avoid cycles of freezing and thawing.
• Shipping: Ambient