Rabbit IgG (Fc specific adsorbed F(ab)2 Fragment) Goat Polyclonal Antibody

SKU R1411HRP

Rabbit IgG (Fc specific adsorbed F(ab)2 Fragment) goat polyclonal antibody, HRP

Product Data

• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.; Recommended Dilutions: This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
• Reactivity: Rabbit
• Antibody Host: Goat
• Clonality: Polyclonal
• Immunogen: Rabbit IgG F(c) fragment.
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.; Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum.; No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Rabbit IgG F(ab')2.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative.; Label: HRP; State: Lyophilized F(ab')2 fragments.; Label: Horseradish Peroxidase (Molecular Weight 40,000 daltons)
• Reconstitution Method: Restore with 0.5 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Immunoaffinity chromatography.
• Conjugation: HRP
• Storage: Store vial at 2-8°C prior to restoration. Restore with 0.5 ml of deionized water (or equivalent). For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.; This antibody is stable for one month at 2-8°C as an undiluted liquid.; Dilute only prior to immediate use.; Avoid repeated freezing and thawing.
• Shipping: Ambient