Mouse IgA (H+L chain), F(ab)2 Fragment, adsorbed Goat Polyclonal Antibody

SKU R1409B

Mouse IgA (H+L chain), F(ab)2 Fragment, adsorbed goat polyclonal antibody, Biotin

Product Data

• Recommended Dilution: Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.; Recommended Dilutions:; ELISA: 1/5,000-1/20,000; Western Blot: 1/500-1/2,000; Immunohistochemistry: 1/500-1/2,000.
• Reactivity: Mouse
• Antibody Host: Goat
• Clonality: Polyclonal
• Immunogen: Mouse IgG, IgA and IgM whole molecules.
• Specificity: This product was prepared from polyspecific antiserum by Immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.; Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Goat Serum.; No reaction was observed against Human Serum proteins.; This product is suitable for the detection of all Mouse immunoglobulin classes, isotypes and chain combinations.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.; Label: Biotin; State: Lyophilized F(ab')2 fragments.; Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC); Molar radio: 10-20 BAC molecules per Goat IgG molecule.
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Immunoaffinity chromatography.
• Conjugation: Biotin
• Storage: Store vial at 2-8°C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.; This antibody is stable for one month at 2-8°C as an undiluted liquid.; Dilute only prior to immediate use.; Avoid repeated freezing and thawing.
• Shipping: Ambient