Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed Goat Polyclonal Antibody
Product Data | |
Application | ELISA, WB |
---|---|
Recommended Dilution | ELISA: 1/20000. Western Blot: 1/500-1/2000. Immunohistochemistry: 1/250-1/1000. |
Reactivity | Mouse |
Antibody Host | Goat |
Clonality | Polyclonal |
Immunogen | Mouse IgG F(c) fragment. |
Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(ab’)2 or Bovine, Horse, and Human Serum Proteins. |
Buffer | 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 Label: HRP State: Lyophilized F(ab')2 fragments Stabilizer: 10 mg/ml BSA (IgG and Protease free) Preservative: 0.01% (w/v) Gentamicin Sulfate Label: Horseradish Peroxidase (Molecular Weight 40,000 daltons) |
Reconstitution Method | Restore with 1.0 ml of deionized water (or equivalent). |
Concentration | lot specific |
Purification | Immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation |
Conjugation | HRP |
Storage | Prior to reconstitution store at 2-8°C. |
Shipping | Ambient |
Note | Do Not add Sodium Azide! |
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