Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed Goat Polyclonal Antibody

SKU R1406B

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, Biotin

Product Data

• Application: ELISA, WB
• Recommended Dilution: Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.; Recommended Dilutions:; ELISA: 1/20,000-1/100,000.; Western blot: 1/2,000-1/10,000.; Immunohistochemistry: 1/1,000-1/5,000.; Note: This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitution concentration is suggested for this product.
• Reactivity: Mouse
• Antibody Host: Goat
• Clonality: Polyclonal
• Immunogen: Mouse IgG F(c) fragment.
• Specificity: Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum.; No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(ab’)2 or Bovine, Horse and Human Serum Proteins.
• Buffer: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2; Label: Biotin; State: Lyophilized F(ab')2 fragments; Stabilizer: 10 mg/ml BSA (IgG and Protease free); Preservative: 0.01% (w/v) Sodium Azide; Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC); Molar radio: 10-20 BAC molecules per Goat IgG molecule.
• Reconstitution Method: Restore with 0.5 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
• Conjugation: Biotin
• Storage:

  Prior to reconstitution store at 2-8°C.; Following reconstitution store undiluted at 2-8°C for one month; or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.

• Shipping: Ambient