Feline IgG (H+L chain) Rabbit Polyclonal Antibody

SKU R1307B

Feline IgG (H+L chain) rabbit polyclonal antibody, Biotin

Product Data

• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoelectron microscopy and Immunohistochemistry.; Recommended Dilutions: This product has been assayed against 1.0 ug of Cat IgG in a standard capture ELISA using ABTS as a substrate. A working dilution of 1:27,000 to 1:32,000 of the reconstitution concentration is suggested for this product.
• Reactivity: Feline
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Feline IgG whole molecule.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Cat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.; Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Cat IgG and Cat Serum.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Thimerosal as preservative.; Label: Biotin; State: Lyophilized purified Ig fraction.; Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC); Molar radio: 10-20 BAC molecules per Rabbit IgG molecule.
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Purification: Immunoaffinity chromatography.
• Conjugation: Biotin
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.; This antibody is stable for one month at 2-8°C as an undiluted liquid.; Dilute only prior to immediate use.; Avoid repeated freezing and thawing.
• Shipping: Ambient