Bovine IgG (H+L chain) Rabbit Polyclonal Antibody

SKU R1304B

Bovine IgG (H+L chain) rabbit polyclonal antibody, Biotin

Product Data

• Application: ELISA, IF, IHC, WB
• Recommended Dilution: Immunoblotting (1/2,000-1/10,000).; ELISA (1/20,000-1/100,000).; Immunohistochemistry (1/1,000-1/5,000).; Immunomicroscopy.; as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. ; This product has been assayed against 1.0 µg of Bovine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000-1/20,000 of the reconstitution concentration is suggested for this product.
• Reactivity: Bovine
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Bovine IgG whole molecule
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. ; Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Bovine IgG and Bovine Serum.
• Buffer: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2; Label: Biotin; State: Lyophilized purified IgG fraction; Stabilizer: 10 mg/ml Polyethylene Glycol (PEG-8000); Preservative: 0.01% (w/v) Sodium Azide; Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC); Molar radio: 10-20 BAC molecules per Goat IgG molecule.
• Reconstitution Method: Restore with deionized water (or equivalent)
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.; After reconstitution store the antibody undiluted at 2-8°C for one month ; or (in aliquots) at -20°C long term.; Avoid repeated freezing and thawing.
• Shipping: Ambient