Goat IgG (H+L chain), adsorbed Donkey Polyclonal Antibody

SKU R1254F

Goat IgG (H+L chain), adsorbed donkey polyclonal antibody, FITC

Product Data

• Recommended Dilution: Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.; Recommended Dilutions:; FLISA: 1/10,000-1/50,000.; IF Microscopy: 1/1,000-1/5,000.; Flow Cytometry: 1/500-1/2,500.
• Reactivity: Goat
• Antibody Host: Donkey
• Clonality: Polyclonal
• Immunogen: Goat IgG whole molecule.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.; Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Donkey Serum, Goat IgG and Goat Serum.; No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.; Label: FITC; State: Lyophilized purified Ig fraction.; Label: Fluorescein isothiocyanate , Molecular Weight 390 daltons); Absorption emission: 495 nm / 528 nm; Molar radio: 3.9 moles FITC per mole of Donkey IgG.
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography.
• Conjugation: FITC
• Storage: Store vial at 2-8°C prior to restoration.; Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for long term storage.; Dilute only prior to immediate use.; Avoid repeated freezing and thawing.
• Shipping: Ambient