Mouse IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: R1253HRP

Mouse IgG (H+L chain) rabbit polyclonal antibody, HRP

Product Images

Western Blot of Mouse IgG Antibody Peroxidase Conjugated detecting mouse IgG. Mouse IgG (1.0 mg) was separated on a 4-20% gradient gel and then transferred to Whatman Protran BA 85 nitrocellulose (0.45 µm pore size) and blocked with 5% BLOTTO (p/n [B501-0500]) o/n at 4°C. The membrane was then probed with a 1/2,500 dilution of HRP Rabbit anti-Mouse IgG [H&L] R1253HRP for 45 min at RT. FemtoMax Super Sensitive Chemiluminescent HRP Substrate Was added and the image was captured using a BioSpectrum Imaging System with a BioChemi 500 -28C cooled 4.0 Megapixel CCD Camera from UVP (www.uvp.com, Upland, CA). Binning: 5X5. Exposure time: 2 min. Aperture open. No emission or excitation filters used.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
  Recommended Dilutions:
  ELISA : 1:50,000 - 1:300,000
  Western Blot : 1:10,000 - 1:30,000
  Immunohistochemistry: 1:500 - 1:2,500
  Other Dilution: User Optimized
• Reactivities: Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Mouse IgG whole molecule.
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
  Label: HRP
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (IgG and Protease free)
  Preservative: 0.01% (w/v) Gentamicin Sulfate
  Label: Horseradish Peroxidase
  Conjugation Chemistry: Periodate
• Reconstitution Method: Reconstitute with 1.0 ml of deionized water (or equivalent).
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography using Mouse IgG coupled to agarose beads
• Conjugation: HRP
• Storage: Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
  For extended storage aliquot and freeze at -20°C or below.
  Avoid cycles of freezing and thawing! Dilute only prior to immediate use!