Mouse IgG (H+L chain) Rabbit Polyclonal Antibody

SKU R1253B

Mouse IgG (H+L chain) rabbit polyclonal antibody, Biotin

Product Data

• Application: ELISA, IHC, WB
• Recommended Dilution: Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.; Recommended Dilutions:; ELISA: 1/300,000.; Western blot: 1/2,000-1/10,000.; Immunohistochemistry: 1/1,000-1/5,000.
• Reactivity: Mouse
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Mouse IgG whole molecule.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.; Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
• Buffer: 0.01M Sodium Phosphate, 0.14M Sodium Chloride, pH 7.4; Label: Biotin; State: Lyohilized purified Ig fraction; Stabilizer: 10 mg/ml BSA (IgG and Protease free); Preservative: 0.01% (w/v) Sodium Azide; Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC); Molar radio: 10-20 BAC molecules per Rabbit IgG molecule
• Reconstitution Method: Reconstitute with 1.0 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Conjugation: Biotin
• Storage: Store secondary antibody at 4° C prior to reconstitution. ; Centrifuge product if not completely clear after standing at room temperature. ; This product is stable for several weeks at 4° C as an undiluted liquid. ; Dilute only prior to immediate use. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing!
• Shipping: Blue Ice