LGR5 Mouse Monoclonal Antibody [Clone ID: UMAB212]

SKU UM800104CF

Carrier-free (BSA/glycerol-free) LGR5 mouse monoclonal antibody,clone UMAB212

Product Data

• Clone Name: UMAB212
• Application: 10k-ChIP, FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000~2000, IHC 1:100~150 (recommended antigen retrieval buffer: TEE buffer pH 9.0), IF 1:100, FLOW 1:100
• Reactivity: Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 22-543 of human LGR5 (NP_003658) produced in HEK293 cell.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 99.8 kDa
• Gene Name: leucine rich repeat containing G protein-coupled receptor 5
• Database Link:
  NP_003658
  O75473
• Synonyms: FEX; GPR49; GPR67; GRP49; HG38

Reference Data

• Protein Families: Druggable Genome, GPCR, Transmembrane