MGMT Mouse Monoclonal Antibody [Clone ID: UMAB56]

CAT#: UM800007CF

Carrier-free (BSA/glycerol-free) MGMT mouse monoclonal antibody,clone UMAB56

Product Images

Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-MGMT mouse monoclonal antibody. (UM800007; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human lung tissue using anti-MGMT mouse monoclonal antibody. (UM800007; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MGMT mouse monoclonal antibody. (UM800007; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-MGMT mouse monoclonal antibody. (UM800007; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MGMT mouse monoclonal antibody. (UM800007; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MGMT mouse monoclonal antibody. (UM800007; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-MGMT mouse monoclonal antibody. (UM800007; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human tonsil using anti-MGMT mouse monoclonal antibody. (UM800007; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

• HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MGMT (RC229131, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MGMT (1:500).

• Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MGMT monoclonal antibody (Clone UMAB56).

• Immunofluorescent staining of HeLa cells using anti-MGMT mouse monoclonal antibody (UM800007, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).

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Specifications

Product Data

• Clone Name: UMAB56
• Applications: IF, IHC, WB
• Recommended Dilution: IHC 1:50~100, IF 1:100
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MGMT (NP_002403) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens O-6-methylguanine-DNA methyltransferase (MGMT), mRNA.
• Database Link:
  NP_002403
  Entrez Gene 4255 Human
  UniProt ID P16455
• Synonyms: methylguanine-DNA methyltransferase; O-6-methylguanine-DNA methyltransferase; O6-methylguanine-DNA methyltransferase; OTTHUMP00000020741

Reference Data

• Protein Families: Druggable Genome