SIRT1 Mouse Monoclonal Antibody [Clone ID: 1F3]

SKU TA336801

Mouse Monoclonal Sirtuin 1/SIRT1 Antibody (1F3)

Product Data

• Clone Name: 1F3
• Application: ELISA, FC, ICC/IF, IHC, WB
• Recommended Dilution: Flow (Intracellular), Immunohistochemistry Free-Floating, Flow Cytometry: 1 ug per million cells, ELISA: 1:10000, Immunohistochemistry: 1:200-1:1000, Immunocytochemistry/ Immunofluorescence: 1:10-1:1000, Immunohistochemistry-Paraffin: 1:200-1:1000, Western Blot: 1:500-1:2000
• Reactivity: Human, Mouse, Primate, Rat
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human SIRT1 expressed in E. coli. [UniProt# Q96EB6]
• Buffer: PBS, 0.03% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Ammonium sulfate precipitation
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Predicted Protein Size: 120 kDa
• Gene Name: sirtuin 1
• Database Link:
  NP_036370
  Q96EB6
• Background: SIRT1, the human homolog of the S. cerevisiae Sir2 protein, functions as an NAD-dependent deacetylase of a number of nonhistone substrates including p53. In response to DNA damage, SIRT1 binds and deacetylates the p53 protein at c-terminal Lys382 residue and attenuate p53-mediated functions. When overexpressed in mouse embryo fibroblasts, SIRT1 antagonizes PML-induced acetylation of p53 and rescues PML-mediated premature cellular senescence. In mammalian cells, SIRT1 appears to control the cellular response to stress by regulating the FOXO family of forkhead transcription factors.
• Synonyms: SIR2; SIR2alpha; SIR2L1
• Note: This SIRT1 (1F3) antibody is useful for Western blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and ELISA.

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors