Apolipoprotein E (APOE) Mouse Monoclonal Antibody [Clone ID: WUE-4]

SKU TA336538

Mouse Monoclonal Apolipoprotein E/ApoE Antibody (WUE-4)

Product Data

• Clone Name: WUE-4
• Application: CyTOF-ready, ELISA, FC, ICC/IF, IHC, IP, WB
• Recommended Dilution: Flow (Intracellular), Immunohistochemistry-Paraffin, Flow Cytometry: 1 ug per million cells, ELISA: 1:100-1:2000, Immunohistochemistry: 1:50-1:200, Immunoprecipitation: 1:10-1:500, Western Blot: 2 ug/ml, Immunocytochemistry/ Immunofluorescence: 1:200, CyTOF-ready
• Reactivity: Human, Mouse (Does not react with: Rat)
• Antibody Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: Purified human ApoE [UniProt# P02649]
• Buffer: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Predicted Protein Size: 36 kDa
• Gene Name: apolipoprotein E
• Database Link:
  NP_000032
  P02649
• Background: ApoE (apolipoprotein E) is the protein constituent of cholesterol/triglyceride-rich plasma lipoproteins, and is a multifunctional glycosylated secretary protein found almost in all organs with high activity in hepatic tissues. ApoE expression is induced by cholesterol-rich diets and is enhanced in lipoproteins in humans with genetic disorder type III hyperlipoproteinemia (HLP) characterized by remnant lipoproteins accumulation in plasma and premature atherosclerosis. ApoE circulates in blood as a protein component of VLDLs, chylomicron remnants, a subclass of HDL etc, and in cerebrospinal fluid as well as CNS interstitial fluid on small particles and disks resembling HDLs. ApoE facilitates transport of cholesterol and other lipids, as well as the clearance of plasma lipoproteins by serving as a critical ligand for lipoprotein uptake by LDL receptors and related proteins. ApoE participates in lipids redistribution to cells (e.g. CNS) that require cholesterol and phospholipids for reparative processes. ApoE also involves in proliferation inhibition of smooth muscle cells/lymphocytes, antigen presentation, and cholesterol efflux stimulation from foam cell macrophages. Defects in APOE have been linked to HLPP3 (hyperlipoproteinemia type 3), AD2 (Alzheimer disease type 2), SBHD (sea-blue histiocyte disease), LPG (lipoprotein glomerulopathy), and certain autoimmune disorders including multiple sclerosis and psoriasis.
• Synonyms: AD2; APO-E; LDLCQ5; LPG
• Note: This ApoE antibody is useful for Western blot, ELISA, Immunohistochemistry and Immunoprecipitation. In Western blot a band is observed at ~36 kDa, representing the ApoE protein.

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, Secreted Protein, Stem cell - Pluripotency
• Protein Pathways: Alzheimer's disease