LOX Rabbit Polyclonal Antibody

SKU TA336520

Rabbit Polyclonal LOX propeptide Antibody

Product Data

• Application: ICC/IF, IHC, Immunoblotting, WB
• Recommended Dilution: Western Blot: 1:500-1:2000, Immunohistochemistry: 0.5-1.0 ug/ml, Immunohistochemistry-Paraffin: 1:100-1:250, Immunocytochemistry/ Immunofluorescence: 5 ug/ml, Immunoblotting
• Reactivity: Human, Mouse, Porcine, Primate, Rat
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to an internal region of mouse LOX propeptide (residues 78-115). [UniProt# P28301]
• Buffer: PBS, 0.02% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Gene Name: lysyl oxidase
• Database Link:
  NP_002308
  P28300
• Background: Lysyl oxidase (LOX) is a critical enzyme in extracellular matrix biosynthesis as well as in the maintainance of normal bone phenotype. It is synthesized/secreted as a 50-kD proenzyme 'pro-LOX' which is then processed to 30-kD mature enzyme 'LOX' and 18-kD lysyl oxidase propeptide 'LOX-PP' by extracellular procollagen C-proteinases (BMP1, Tll1, and Tll2). LOX-PP has been proposed to implicate in the maintenance of inactive state of LOX within the secretory pathway and to act as intramolecular chaperones which facilitate the accurate protein folding/targeting to correct destinations. LOX-PP involves tumor suppression by inhibiting RAS-dependent cellular transformation and its expression has also been associated with inhibition of anchorage-independent progression as well as migration and growth suppression xenografts in Her-2/neu-driven breast cancer. LOX-PP also inhibits PI3K/AKT and ERK1/2 MAP kinase pathways, as well as the levels of downstream NF-kB/cyclin D1 signals in certain cancers - breast, pancreatic, lung, prostate and oral cancers. LOX-PP has also been shown to inhibit DNA synthesis in rat's VSMCs cultures, osteoblast proliferation and FGF-2 signaling.
• Synonyms: MGC105112
• Note: This LOX propeptide antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin and Western Blot where a band is seen at ~18 kDa. The strong band at 50 kDa and smear of bands that run between 46 kDa and 50 kDa are glycosylated and non-glycosylated forms of pro-lysyl oxidase. Bands between 28-36 kDa are glycosylated propeptide. The variation in MW depends on the extent of glycosylation.

Reference Data

• Protein Families: Druggable Genome, Secreted Protein