IKB alpha (NFKBIA) Mouse Monoclonal Antibody [Clone ID: 6A920]

SKU TA336372

Mouse Monoclonal IkB-alpha Antibody (6A920)

Product Data

• Clone Name: 6A920
• Application: CyTOF-ready, FC, ICC/IF, IHC, IP, Simple Western, WB
• Recommended Dilution: Flow Cytometry: 0.25-1 ug/10^6 cells, Immunoprecipitation: 1 ug/ml, Western Blot: 1-2 ug/ml, Immunohistochemistry: 1:20-1:1000, Simple Western: 1:20, Immunohistochemistry-Paraffin: 1:100, Immunocytochemistry/ Immunofluorescence: 1:100-1:1000, Knockout Validated, CyTOF-ready
• Reactivity: Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: A recombinant protein corresponding to amino acid residues 32-291 of human IkBa was used as immunogen.
• Buffer: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Gene Name: NFKB inhibitor alpha
• Database Link:
  NP_065390
  P25963
• Background: NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first gene induced following NF-kB activation is IkBa.
• Synonyms: IKBA; MAD-3; NFKBI

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pathways in cancer, Prostate cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway