HMG1 (HMGB1) Rabbit Polyclonal Antibody

SKU TA301448

Rabbit Polyclonal Antibody against HMGB1 - Oligodendrocyte Marker

Product Data

• Application: ELISA, FC, ICC/IF, IHC, Simple Western, WB
• Recommended Dilution: Simple Western: 1:2000, Flow Cytometry: 1:100, Flow (Intracellular), ELISA, Western Blot: 0.5-1.0 ug/ml, Immunocytochemistry/ Immunofluorescence: 0.05 ug/ml, Immunohistochemistry: 1:100-1:250, Immunohistochemistry-Paraffin: 1:100-1:250, Knockdown Validated
• Reactivity: Bovine, Dog, Hamster, Human, Mouse, Rat
• Antibody Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to an internal portion of the human HMGB1 protein sequence (between residues 100-200). [UniProt #P09429]
• Buffer: Tris-glycine, 150mM NaCl and 0.1% sodium azide
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Gene Name: high mobility group box 1
• Database Link:
  NP_002119
  P09429
• Background: HMGB1 and HMGB2 are part of the chromatin non-histone high mobility group proteins 1 and 2. These proteins (containing multiple HMG-boxes) are conserved domains of 80 amino acids which mediate the DNA binding of many proteins. HMG box domains recognize DNA structure. Both HMGB1 and HMGB2 contain an N-terminal HMG box, a central HMG box, and an acidic carboxy terminus. The acidic tails of these proteins contain multiple serine residues which match the phosphorylation consensus sites of casein kinase II, and phosphorylation of this domain appears to be important for proper functioning of these proteins. HMGB1 and HMGB2 have been shown to facilitate the binding of various sequence-specific transcription factors to their respective DNA binding sites. They may also serve as architectural factors that recognize and mediate DNA structural changes that accompany various events such as DNA repair, transcription, and replication.
• Synonyms: HMG1; HMG3; SBP-1

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Base excision repair