Desmin (DES) Mouse Monoclonal Antibody [Clone ID: OTI4G1]

CAT#: CF502328

Carrier-free (BSA/glycerol-free) DES mouse monoclonal antibody, clone OTI4G1 (formerly 4G1)

Product Images

HEK293T cells transfected with either RC205685 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DES antibody (TA502328), and then analyzed by flow cytometry.

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DES (Cat# RC205685, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DES(Cat# TA502328). Positive lysates LY400713 (100ug) and LC400713 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DES mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502328)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-DES mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502328)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-DES mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502328)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-DES mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502328)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-DES mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502328)

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Specifications

Product Data

• Clone Name: OTI4G1
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:200~500, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human fragment corresponding to amino acids 104-391 of human DES (NP_001918) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53.4 kDa
• Gene Name: Homo sapiens desmin (DES), transcript variant 1, mRNA.
• Database Link:
  NP_001918
  Entrez Gene 13346 MouseEntrez Gene 64362 RatEntrez Gene 1674 Human
  UniProt ID P17661
• Background: This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. [provided by RefSeq, Jul 2008]
• Synonyms: CDCD3; CSM1; CSM2; LGMD1D; LGMD1E; LGMD2R

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM)