CD300 antigen (CD300A) Mouse Monoclonal Antibody [Clone ID: MEM-260]
$335.00
2 Weeks*
Specifications
| Product Data | |
| Clone Name | MEM-260 |
|---|---|
| Application | FC, WB |
| Recommended Dilution | Flow Cytometry: 2 µg/ml. Western Blot (Non-reducing conditions). Positive material: HPB cell line; Negative material: JURKAT human cell line, RAJI human cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | HPB human acute lymphoid leukemia cell line |
| Specificity | This antibody reacts with CD300a, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25% of circulating T cells and 15% of circulating B cells. |
| Buffer | PBS, pH 7.4 containing 15 mM sodium azide as preservative State: Purified State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE) |
| Concentration | lot specific |
| Purification | Protein A affinity chromatography |
| Conjugation | Unconjugated |
| Storage | Store undiluted at 2-8°C. DO NOT FREEZE! |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | CD300a molecule |
| Database Link | |
| Background | CD300a (CMRF-35H, IRp60) is a non-MHC-specific inhibitory receptor of immunoglobulin superfamily, which contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that associate with SH2-containing phosphatases SHP-1 and SHP-2. CD300a is expressed on many cell types including T cells, NK cells, neutrophils, eosinophils or mast cells. Its triggering inhibits activating signals such as those of IL5, GM-CSF or eotaxin, as well as supresses mast cell degranulation or NK cell cytotoxic activity. |
| Synonyms | CLM-8, CMRF35-H, CMRF35-H9, CMRF-35-H9, IRC1/IRC2, IRp60 |
| Reference Data | |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.